Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA137797 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Nontypeable Haemophilus influenzae (NTHi) is a common causative organism of acute otitis media (AOM) in children. A human cDNA microarray comprising 30,968 human genome probes was used to evaluate the transcriptional changes that occur in peripheral blood mononuclear cells (PBMC) at the onset of clinical AOM caused by NTHi infection in children after comparison of microarray results with the pre-infection healthy stage of the same children.
2012-03-15 | GSE27990 | GEO
Project description:Nontypeable Haemophilus influenzae (NTHi) is a common causative organism of acute otitis media (AOM) in children. A human cDNA microarray comprising 30,968 human genome probes was used to evaluate the transcriptional changes that occur in peripheral blood mononuclear cells (PBMC) at the onset of clinical AOM caused by NTHi infection in children after comparison of microarray results with the pre-infection healthy stage of the same children. Four to ten milliliters of heparinized peripheral venous blood was collected from children at 6 to 30 months of age when they were in acute otitis media (AOM) stage and pre-infection healthy stage. The diagnosis of AOM was based on symptoms and signs as well as Nontypeable Haemophilus influenzae culture positive in the middle ear fluid. Patients with polymicrobial infections, history of immunodeficiency, history of chronic or recurrent AOM, chronic disease, or receiving steroids or other immunomodulatory agents were excluded. Peripheral blood mononuclear cells (PBMCs) were isolated by the Ficoll gradient and total RNA was extracted from PBMCs using the QIAamp RNA blood Mini Kit (Qiagen, Maryland, USA) according to manufacturerM-bM-^@M-^Ys instructions. Double-stranded cDNA generated from total RNA was labeled with Cyanine-5 and subsequently hybridized to Human OneArray glass slides according to the manufacturer's standard protocols (PhalanxBio Inc, CA, USA). Microarrays were scanned at 5 M-NM-<m resolution using an Agilent scanner. Raw intensity signals for each microarray were captured using a Molecular Dynamics Axon 4100A scanner, measured using GenePixProM-bM-^DM-" Software. The data from all microarrays in each experimental set was then analyzed using Omicsoft Array Studio software; control and missing features were removed, and the remaining signals were quantile normalized.
2012-03-15 | E-GEOD-27990 | biostudies-arrayexpress
Project description:Streptococcus pneumoniae (Spn) is the predominant causative organism of acute otitis media (AOM) in children. A human cDNA microarray comprising 30,968 human genome probes was used to evaluate the transcriptional changes that occur in peripheral blood mononuclear cells (PBMC) at the onset of clinical AOM caused by Spn infection in children after comparison of microarray results with the pre-infection healthy stage of the same children.
2010-07-26 | GSE23140 | GEO
Project description:Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 4 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(4):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012.
2013-12-13 | GSE53287 | GEO
Project description:Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 7 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(7):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012.
2013-12-13 | GSE53289 | GEO
Project description:Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 4 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(4):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012. R2866 wild-type (n=4) and R2866Δ0112 mutant (n=4) were grown for 4 hours in supplemented brain-heart infusrion medium The 4hr and 7hr analyses were performed separately and, therefore, the background corrections are different between the two experiments.
2013-12-13 | E-GEOD-53287 | biostudies-arrayexpress
Project description:Gene expresison profiles of the R2866 WT and R2866Δ0112 mutant gorwn 7 hours in supplemented brain-heart infusion medium. This experiment was conducted to determine the effects of R2866_0112 gene deletion on overall gene expression profile. The results from this study are further discussed in: Modified lipooligosaccharide structure protects nontypeable Haemophilus influenzae from IgM-mediated complement killing in experimental otitis media. Langereis JD, Stol K, Schweda EK, Twelkmeyer B, Bootsma HJ, de Vries SP, Burghout P, Diavatopoulos DA, Hermans PW. MBio. 2012 Jul 3;3(7):e00079-12. doi: 10.1128/mBio.00079-12. Print 2012. R2866 wild-type (n=4) and R2866Δ0112 mutant (n=4) were grown for 7 hours in supplemented brain-heart infusrion medium The 4hr and 7hr analyses were performed separately and, therefore, the background corrections are different between the two experiments.
2013-12-13 | E-GEOD-53289 | biostudies-arrayexpress
Project description:Streptococcus pneumoniae (Spn) is the predominant causative organism of acute otitis media (AOM) in children. A human cDNA microarray comprising 30,968 human genome probes was used to evaluate the transcriptional changes that occur in peripheral blood mononuclear cells (PBMC) at the onset of clinical AOM caused by Spn infection in children after comparison of microarray results with the pre-infection healthy stage of the same children. Four to ten milliliters of heparinized peripheral venous blood was collected from children at 6 to 30 months of age when they were in acute otitis media (AOM) stage and pre-infection healthy stage. The diagnosis of AOM was based on symptoms and signs as well as S. pneumoniae culture positive in the middle ear fluid. Patients with polymicrobial infections, history of immunodeficiency, history of chronic or recurrent AOM, chronic disease, or receiving steroids or other immunomodulatory agents were excluded. Peripheral blood mononuclear cells (PBMCs) were isolated by the Ficoll gradient and total RNA was extracted from PBMCs using the QIAamp RNA blood Mini Kit (Qiagen, Maryland, USA) according to manufacturer’s instructions. Double-stranded cDNA generated from total RNA was labeled with Cyanine-5 and subsequently hybridized to Human OneArray glass slides according to the manufacturer's standard protocols (PhalanxBio Inc, CA, USA). Microarrays were scanned at 5 μm resolution using an Agilent scanner. Raw intensity signals for each microarray were captured using a Molecular Dynamics Axon 4100A scanner, measured using GenePixPro™ Software. The data from all microarrays in each experimental set was then analyzed using Omicsoft Array Studio software; control and missing features were removed, and the remaining signals were quantile normalized.
2010-07-26 | E-GEOD-23140 | biostudies-arrayexpress
Project description:Chronic Otitis Media (OM) develops after sustained inflammation and is characterized by secretory middle ear epithelial metaplasia and effusion, most frequently mucoid. Non-typeable Haemophilus influenzae (NTHi), the most common acute OM pathogen, is known to activate inflammation and mucin expression in vitro and in animal models of OM. The goals of this study were to: examine expression profiling epithelial effects of NTHi challenge in murine middle ears. We used microarrays to detail examine the global programme of gene expression underlying epithelial effects of NTHi challenge in murine middle ears during this study.
2012-11-13 | GSE40087 | GEO
Project description:Transcriptome profile of peripheral blood mononuclear cells in children with acute otitis media caused by Streptococcus pneumoniae
| PRJNA131705 | ENA