Project description:We used profiling of Arabidopsis small RNA populations present in the mature ovules to define the potential genome targets of small RNAs during reproductive development. Defining the contributions and interactions of paternal and maternal genomes during embryo development is critical to understand the fundamental processes involved in hybrid vigor, hybrid sterility, and reproductive isolation. To determine the parental contributions and their regulation during Arabidopsis embryogenesis we combined deep-sequencing-based RNA profiling and genetic analyses. At the 2-4 cell stage there is a strong, genome-wide dominance of maternal transcripts, although transcripts are contributed by both parental genomes. At the globular stage the relative paternal contribution is higher, largely due to a gradual activation of the paternal genome. We identified two antagonistic maternal pathways that control these parental contributions. Paternal alleles are initially down-regulated by the chromatin siRNA pathway, linked to DNA and histone methylation, while transcriptional activation requires maternal activity of the histone chaperone complex CAF1. Our results define maternal epigenetic pathways controlling the parental contributions in plant embryos, which are distinct from those regulating genomic imprinting. Profiling of small RNAs in wild type ovules of Arabidopsis

2011-05-15 | E-GEOD-28627 | biostudies-arrayexpress

Arabidopsis thaliana

Project description:Genome-wide profiling of small RNAs in Arabidopsis under abiotic stresses

| PRJNA136741 | ENA

Arabidopsis thaliana

Project description:Arabidopsis thaliana Col-0 inflorescence small RNAs

| PRJNA314979 | ENA

Arabidopsis thaliana Col-0 inflorescence small RNAs

Project description:Small RNA sequences from Arabidopsis thaliana Col-0 inflorescence tissues of three biological replicates. The data were analyzed to identify non-templated nucleotides in Arabidopsis small RNAs.

2016-05-18 | GSE79119 | GEO

Arabidopsis thaliana

Project description:Arabidopsis small RNAs associated with RDR6-dependent RNA-directed DNA Methylation

| PRJNA178113 | ENA