Ovis aries
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ABSTRACT:
PROVIDER: PRJNA138877 | ENA |
REPOSITORIES: ENA
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Project description:The aim of the research was to investigate the regulation of gene expression in ovine blood leukocytes during ACTH challenge and the effect of effects of dietary administration of botanicals to modulate the stress related response. Six homogeneous groups of Sarda sheep were used, one negative control (CT) and five injected with ACTH to mimic biological stress. Afterwards four of the five ACTH treated groups were separately and fed with extract from Echinacea angustifolia roots (PO), with extract from Echinacea angustifolia flowers (EA), with extracts from Andrographis paniculata (AP) and with Larix decidua sawdust (LB). Control groups (CT and ACTH) were fed with a basal diet. RNA was extracted from blood samples collected before (T0) and after 3 (T3) and 51 (T51) hours from the first ACTH injection and transcriptome analysis was performed using a custom oligoarray. The injection of ACTH caused a down regulation of transcripts (95.5%, 232 over 243; P<0.01) at T3, whilst the administration with the diet of AP, EA, LB and PO determined a predominant up regulation. At T51, the down regulated transcripts of the ACTH group in comparison to CT group were lower (36.2%, 142 over 392; P<0.01), whilst for the animals receiving plant extracts with the diet the percentage of down regulated transcripts increased, in particular for LB group (85.8 %). Biological pathways affected by the injection of the adrenocorticotrophic hormone include the MAPK signaling pathway, the p53 signaling pathway, the TGF-beta signaling pathway and the T-cell receptor signaling pathway. The most promising botanicals for the control of induced stress appeared Echinacea angustifolia and Larix decidua, in particular both showing a target for the NR4A1 gene, a glucocorticoid receptor involved in the cell trafficking after cortisol secretion. The RNA extracted from blood samples and depleted from globinic mRNA was used for the preparation of pools. Samples were pooled together within group (6 groups) and time of sampling (3 times), including in the pool an equal quantity of RNA for each sheep. Eighteen pools were obtained for the hybridizations, that were performed in duplicate (36 slides).
2012-12-31 | E-GEOD-28667 | biostudies-arrayexpress
Project description:The aim of the research was to investigate the regulation of gene expression in ovine blood leukocytes during ACTH challenge and the effect of effects of dietary administration of botanicals to modulate the stress related response. Six homogeneous groups of Sarda sheep were used, one negative control (CT) and five injected with ACTH to mimic biological stress. Afterwards four of the five ACTH treated groups were separately and fed with extract from Echinacea angustifolia roots (PO), with extract from Echinacea angustifolia flowers (EA), with extracts from Andrographis paniculata (AP) and with Larix decidua sawdust (LB). Control groups (CT and ACTH) were fed with a basal diet. RNA was extracted from blood samples collected before (T0) and after 3 (T3) and 51 (T51) hours from the first ACTH injection and transcriptome analysis was performed using a custom oligoarray. The injection of ACTH caused a down regulation of transcripts (95.5%, 232 over 243; P<0.01) at T3, whilst the administration with the diet of AP, EA, LB and PO determined a predominant up regulation. At T51, the down regulated transcripts of the ACTH group in comparison to CT group were lower (36.2%, 142 over 392; P<0.01), whilst for the animals receiving plant extracts with the diet the percentage of down regulated transcripts increased, in particular for LB group (85.8 %). Biological pathways affected by the injection of the adrenocorticotrophic hormone include the MAPK signaling pathway, the p53 signaling pathway, the TGF-beta signaling pathway and the T-cell receptor signaling pathway. The most promising botanicals for the control of induced stress appeared Echinacea angustifolia and Larix decidua, in particular both showing a target for the NR4A1 gene, a glucocorticoid receptor involved in the cell trafficking after cortisol secretion.
2012-12-31 | GSE28667 | GEO
Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1) and performed a detailed survey of gene expression across different tissues. RNA-seq data of 7 tissue types from the reference female Texel and skin tissue from a Gansu alpine fine wool sheep were sequenced.
2014-06-06 | GSE56643 | GEO
Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1).
2014-06-06 | GSE56644 | GEO
Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1) and performed a detailed survey of gene expression across different tissues. RNA-seq data of 7 tissue types from the reference female Texel and skin tissue from a Gansu alpine fine wool sheep were sequenced. Here is the part of the RNA-seq data sequenced in BGI, including 7 tissue types from the reference female Texel and skin type from a Gansu alpine fine wool sheep.
2014-06-06 | E-GEOD-56643 | biostudies-arrayexpress
Project description:We have completed the high quality reference genome for domestic sheep (Oar v3.1). Early-stage Illumina GA sequence platform sequenced less reads in high GC content regions than in other regions. To read through higher GC content regions, we generated 2 Gb MeDIP-seq data for filling gaps in sheep reference genome assembly.
2014-06-06 | E-GEOD-56644 | biostudies-arrayexpress
Project description:In the present study, we studied the effect of dietary selenium (Se) supplementation on the transcriptomic profile of sheep. The main objective was to evaluate the effect of Se-supplementation on the overall transcriptome of sheep, the altered pathways, and the biological processes related to it . A custom oligo microarray platform (AMADID: 070119) was designed, then used to profile gene expression from 20 samples from 10 sheep at two time points (T0; before Se-supplementation, and T40; at the end of a 40-d Se-supplementation period). Isolated and purified total RNAs were individually hybridized to the custom (4x44k) DNA microarray. The comparison of control and treated animal transcriptomes revealed a large set of differentially expressed genes. After functional analysis and qPCR validation, the result showed several pathways and biological processes that have been altered following Se-supplementation to the diet.
2016-04-26 | GSE69997 | GEO
Project description:Gene expression profile of sheep following dietary iodide supplementation
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Project description:Regional Immune Response to Trichostrongylis colubriformis - Gene Expression during Challenge of Immunized Sheep
| PRJNA133175 | ENA
Project description:RNA sequencing of sheep infraspinatus muscle after tendon release and repair under concomitant nandrolone administration
| PRJNA318767 | ENA