Project description:Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries. This form of reproductive diapause is a winter adaptation. Here we used Affymetrix microarrays to study global expression in female heads associated with diapause.
Project description:Young Drosophila females respond to low temperature and short photoperiod by developmental arrest of the ovaries. This form of reproductive diapause is a winter adaptation. Here we used Affymetrix microarrays to study global expression in female heads associated with long vs. short day.
Project description:The study describes differential expression of genes in adult female flies (Canton S) kept for three weeks in reproductive diapause, compared to ones kept in normal conditions. It discusses signal pathways that are activated or inactivated during diapause and how this relates to the general diapause phenotype with arrested vitellogenesis, extended lifespan, decreased somatic senescence, increased stress resistance and reallocated energy resources.
Project description:Expression data for aging female drosophila melanogaster Female drosophila heads were collected RNA extraction and hybridization on Affymetrix microarrays.
Project description:We used long-oligonucleotide microarrays to investigate whether alternative splicing in Drosophila is regulated in a sex-, stage-, or tissue-specific manner. To examine sex-specific splicing, we compared gene expression profiles of male and female pupae 12 hours after pupariation. To examine stage-specific splicing, we compared expression profiles of mixed-sex, 0-24 hour old embryos and mixed-sex, 12 hour old pupae. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens 24-48 hours after eclosion. To examine tissue-specific splicing, we compared expression profiles of adult male heads and abdomens at 24-48 hours after eclosion. Keywords: tissue-specific expression profiles
