Project description:Dr. Fukuda's laboratory focuses on the cellular and molecular biology of cell surface glycoproteins in development and cancer with a secial emphasis on immune and neural systems. Experiment to determine the genes upregulated in natual killer cells specifically recruited to lymph nodes by tumors Genes upregulated in natual killer cells specifically recruited to lymph nodes by tumors were determine via gene expression analysis Mouse RNA from control mouse NK cell, isolated from spleen, and NK cells isolated from B16 melanoma-bearing lymph nodes, was isolated and prepared in 5 paired samples. RNA was labeled and hybridized to the GLYCOv2 array. Resulting Gene expression patterns were analyzed.
Project description:To determine the influence of primary tumors on pre-metastatic lymph nodes, we have employed whole genome microarray expression profiling as a discovery platform to identify gene signatures of B cells from tumor-draining lymph nodes, compared with normal lymph nodes. We subcutaneously inoculated C57BL/6 mice with the 4T1 mammary carcinoma. Two weeks later, tumor-draining lymph nodes were dissociated and B cells (CD19+) were sorted. Lymph nodes B cells from normal mice without tumor bearing were set as controls.
Project description:To determine the influence of primary tumors on pre-metastatic lymph nodes, we have employed whole genome microarray expression profiling as a discovery platform to identify gene signatures of stromal cells from tumor-draining lymph nodes, compared with normal lymph nodes. We subcutaneously inoculated C57BL/6 mice with the 4T1 mammary carcinoma. Two weeks later, tumor-draining lymph nodes were dissociated and stromal cells (CD45-) were sorted. Lymph nodes stromal cells from normal mice without tumor bearing were set as controls.
