Project description:The basidiomycete Ustilago maydis is the causal agent of corn smut disease and induces tumor formation during biotrophic growth in its host plant maize. The Usilago maydis genome harbors a homolog to the GATA transcription factors Nit2 and AreA that act as global regulators of nitrogen catabolite repression in filamentous model fungi Neurospora crassa and Aspergillus nidulans. We aimed at resolving the role of the Ustilago maydis homolog Ncr1 for the utilization of complex nitrogen sources and pathogenicity. Sporidia of the indicated Ustilago maydis strains were grown overnight in ammonium minimal medium (Holliday, 1976) and samples for total RNA extraction were taken 2h after transfer to minimal medium lacking any nitrogen source (-N) during the exponential growth phase to assess those genes that are regulated in response to nitrogen starvation. The solopathogenic strain SG200 (control) and deletion mutants of (Nitrogen catabolite repression1) Ncr1 and (Target of Ncr1) Ton1, both being in the SG200 background, were studied in two independent experiments (one experiment for Ton1). Per strain and experiment, three biological replicate samples were analyzed (except for only biological replicates for Ncr1 in the second experiment).

2012-01-01 | E-GEOD-28916 | biostudies-arrayexpress

RNAseq of Ustilago maydis strain AB31 and AB31_delta_Num1

Project description:To elucidate the role of Num1 (Um01682) in Ustilago maydis, the transcriptome of wild type and Num1 deletion mutants was determined by RNAseq after b-heterodimer induction

2013-12-09 | E-MTAB-1300 | biostudies-arrayexpress

Ustilago maydis

Project description:Transcriptomic analysis of the role of Rim101 in the adaptation of Ustilago maydis to an alkaline environment

| PRJNA231750 | ENA

Ustilago maydis

Project description:Ustilago maydis Genome sequencing and assembly

| PRJNA506543 | ENA

Ustilago maydis

Project description:Rbf1 induced gene expression in Ustilago maydis

| PRJNA123825 | ENA

Time series transcriptional profiling of Ustilago maydis infecting Zea mays

Project description:The basidiomycete fungus Ustilago maydis causes smut disease in maize and has become an important model for elucidating the strategies used for host colonization by biotrophic fungi. In this study, we performed an in-depth transcriptional profiling of the plant-associated development of a cross between U. maydis FB1 and FB2 wildtype strains. The analysis of eight different stages, including the development on the leaf surface, early colonization, tumor induction and spore maturation, offers an unprecedented view of the changes in the fungal transcriptome associated with the passage through the entirely biotrophic life cycle. In our analysis, we focus on fungal metabolism, nutritional strategies, secreted effectors and regulatory networks. Secreted proteins were enriched in three distinct expression modules corresponding to the plant surface, establishment of biotrophy and tumor formation, respectively. These modules are likely the key determinants for U. maydis virulence. With respect to nutrient utilization, we observed that expression of several nutrient transporters was tied to these virulence modules rather than being controlled by nutrient availability. We show that oligopeptide transporters likely involved in nitrogen supply during infection are important virulence determinants. By measuring the intramodular connectivity of transcription factors, we identified potential drivers for the virulence modules. While known components of the b-cascade served as inducers for the plant surface and biotrophy module, we identified a set of yet uncharacterized transcription factors as likely responsible for expression of the tumor module. We demonstrate a crucial role in effector gene expression and tumor formation for one of these transcription factors.

2018-01-11 | GSE103876 | GEO

Transcriptional analysis of the adaptation of Ustilago maydis to grow under nitrogen fixation conditions

Project description:In this study we show the transcriptional analysis after shifting the Ustilago maydis from media with ammonium as a nitrogen source to media lacking ammonium. From these, 49 genes were up-regulated and 41 were down-regulated. The functional description and gene ontology terms associated to the diferentially expressed genes revealed that various key pathways were represented, including, secondary metabolism, the metabolism of nitrogen, amino acid, fatty acid and amino sugar, among others, suggesting that the interplay of U. maydis with its N2 fixing bacterial endosymbiont is a flexible process that may be active during the adaptation to the fungus to the different nitrogen sources, and possibly during its pathogenic style of life.

2017-04-25 | GSE89625 | GEO

Ustilago maydis

Project description:Genome sequencing of Ustilago maydis ITA-Chassis strain

| PRJNA592070 | ENA