Project description:In this study, we examined the consequences of the early stress (ES) of maternal separation on hippocampal gene expression in middle-aged animals. Middle-aged (15 months old) ES animals exhibit hippocampal transcriptome changes with a significant regulation of genes associated with ion binding, transcriptional regulation, cellular projection, cellular stress responsive pathways, neuronal development and chromatin remodeling.
Project description:In this study, we examined the consequences of the early stress (ES) of maternal separation on hippocampal gene expression in middle-aged animals. Middle-aged (15 months old) ES animals exhibit hippocampal transcriptome changes with a significant regulation of genes associated with ion binding, transcriptional regulation, cellular projection, cellular stress responsive pathways, neuronal development and chromatin remodeling. Agilent one-color experiment, Agilent-024724 Genotypic-designed Custom Rattus Norvegicus 8x15k; Organism: Rattus norvegicus; Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442); Biological replicates: 5 per early stress group, 3 per control group
Project description:In this study, we examined the consequences of the early stress (ES) of maternal separation on hippocampal gene expression in young adulthood. Young adult (2 months old) ES animals exhibit hippocampal transcriptome changes with a significant regulation of genes associated with intracellular signaling, MAP kinase signaling, plasma membrane function, neurotransmitter and neuropeptide receptors and cytoskeletal components.
Project description:Aging is believed to be the result of alterations of protein expression and accumulation of changes in biomolecules. Although there are numerous reports demonstrating changes in protein expression in brain during aging, only few of them describe global changes in the protein level. Here, we present a deepest quantitative proteomic analysis of three brain regions, hippocampus, cortex and cerebellum, in mice aged 1 and 12 months, using the total protein approach technique. In all the brain regions, both in young and in middle-aged animals, we identified over 6,700 proteins. We found that although the total protein expression in middle-aged brain structures is practically unaffected by aging, there are significant differences between young adult and middle-aged mice in the expression of some receptors and signaling cascade proteins proven to be significant for learning and memory formation. Our analysis demonstrates that hippocampus is the most unstable structure during natural aging and that the first symptoms of weakening of neuronal plasticity may be observed on protein level in middle-aged animals.
Project description:In this study, we examined the consequences of the early stress (ES) of maternal separation on hippocampal gene expression in young adulthood. Young adult (2 months old) ES animals exhibit hippocampal transcriptome changes with a significant regulation of genes associated with intracellular signaling, MAP kinase signaling, plasma membrane function, neurotransmitter and neuropeptide receptors and cytoskeletal components. Agilent one-color experiment, Agilent-024724 Genotypic-designed Custom Rattus Norvegicus 8x15k; Organism: Rattus norvegicus; Labeling kit: Agilent Quick-Amp labeling Kit (p/n5190-0442); Biological replicates: 4 per treatment group.
Project description:Hippocampal tissues from young and middle-aged C57BL/6J mice were harvested at 4-hour intervals over two days and processed for proteomic analysis using label-free quantification.
Project description:Comparison of the gene expression profiles of adult human brain samples from frontal cortical regions, including samples from young, middle aged, normal aged, and AlzheimerM-bM-^@M-^Ys disease (AD) brains. Comparison of 12 young (<40yr), 9 middle aged (40-70yr), 16 normal aged (70-94yr), and 4 extremely aged (95-106yr)
Project description:Because most human stroke victims are elderly, studies of experimental stroke in the aged rather than the young rat model may be optimal for identifying clinically relevant cellular responses, as well for pinpointing beneficial interventions. We employed the Affymetrix platform to analyze the whole-gene transcriptome following temporary ligation of the middle cerebral artery in aged and young rats. Expression profiling of periinfarcted brain areas of young and aged rats, as well as healthy tissue from naive animals.
Project description:We carried out a global survey of age-related changes in mRNA levels in the C57BL/6NIA mouse hippocampus and found a difference in the hippocampal gene expression profile between 2-month-old young mice and 15-month-old middle-aged mice correlated with an age-related cognitive deficit in hippocampal-based explicit memory formation. Middle-aged mice displayed a mild but specific deficit in spatial memory in the Morris water maze. Keywords: age comparison
