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Danio rerio

ABSTRACT: Raf/MEK signatures in zebrafish liver cells

PROVIDER: PRJNA142819 | ENA |

REPOSITORIES: ENA

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Zebrafish liver tumors

Project description:This SuperSeries is composed of the following subset Series: GSE29307: zebrafish liver tumor signatures GSE29308: Raf/MEK signatures in zebrafish liver cells Refer to individual Series

2011-07-07 | E-GEOD-29351 | biostudies-arrayexpress

Raf/MEK signatures in zebrafish liver cells

Project description:An inducible oncogenic cell line ZFL-ΔRaf1-ER was established, in which oncogenic human Raf-1(ΔRaf1) can be activated in zebrafish liver cells by administration of 4-hydroxytamoxifen (4HT). Transcriptional profilling of the ZFL-ΔRaf1-ER cells with and without administration of 4HT and/or the MEK inhibitor U0126 defined the gene signatures transcriptionally regulated by hyperactive Raf/MEK/MAPK signaling in zebrafish liver cells.

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Project description:zebrafish liver tumor signatures

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Raf/MEK signatures in zebrafish liver cells

Project description:An inducible oncogenic cell line ZFL-?Raf1-ER was established, in which oncogenic human Raf-1(?Raf1) can be activated in zebrafish liver cells by administration of 4-hydroxytamoxifen (4HT). Transcriptional profilling of the ZFL-?Raf1-ER cells with and without administration of 4HT and/or the MEK inhibitor U0126 defined the gene signatures transcriptionally regulated by hyperactive Raf/MEK/MAPK signaling in zebrafish liver cells. The ZFL-?Raf1-ER cells were cultured in 12-well plates till confluence. Cell were starved for 4h in plain medium and susequently treated with or without 1µM 4HT and/or 30µM U0126 for 12h. Biological triplications were taken for each condition.

2011-07-07 | E-GEOD-29308 | biostudies-arrayexpress