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Saccharomyces cerevisiae

ABSTRACT: Effect of AICAr and SAICAr accumulation on global transcription in the ade3 mutant

OTHER RELATED OMICS DATASETS IN: GSE29324

PROVIDER: PRJNA142827 | ENA |

REPOSITORIES: ENA

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Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. These data were compared to those obtained in the ade16ade17 vs WT data set to determine the transcriptional effects associated to AICAR/SAICAR accumulation and those due to ade16 and ade17 deletions. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or lacking AICAR and SAICAR nucleotide derivatives.

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Role of the Bas1p transcription factor in the transcriptional response associated to AICAR and SAICAR accumulation

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Project description:We investigated the role of Pho4p in the transcriptional response associated to AICAR and SAICAR accumulation. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating AICAR and SAICAR in a pho4 deletion context.

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Project description:We investigated the effects of AICAr and SAICAr accumulation on the global transcriptome of yeast, comparing the ade3 mutant to the WT strain.

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Effect of AICAR and SAICAR accumulation on global transcription (first part)

Project description:We investigated the effects of AICAR and SAICAR accumulation on transcriptional regulation of purine biosynthesis and other metabolic pathways. In this work, global transcription analyses were combined to measurements of intracellular nucleotides pools by HPLC. Keywords: Comparative genomic transcription analysis on total RNA from cells accumulating or not AICAR and SAICAR nucleotide derivatives.

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