Project description:ChIP-chip of histone variants and modifications in A. thaliana WT and various mutants In Schizosaccharomyces pombe, H2AZ, H3, H3 acetylated, H3K36me3, H3K4me3 as well as RNAPII (along with associated DNA sequences) were immunoprecipitated. The ChIPs were performed in duplicate from WT cells as well as in few mutants as described below. The extracted DNA was hybridized to a DNA microarray containing an average of 4 probes per kilobase across the whole yeast genome. The combined datasets are available in the supplemental files of the related publication.
Project description:Here, we report the high-throughput profiling of histone modification (H3K9me2) in fission yeast Schizosaccharomyces pombe. We generated genome-wide H3K9me2 maps of fission yeast mutants in swo1-26 (temperature sensitive, ts) cells at 25℃ and 37℃. We find that H3K9me2 enrichment at heterochromatin regions, especially at the mating-type locus and subtelomeres, is compromised, suggesting heterochromatin assembly defects.
