Genomics

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118

Single-tube Linear DNA amplification (LinDA) for robust ChIP-seq


ABSTRACT: Single-tube Linear DNA amplification (LinDA) for robust ChIP-seq

PROVIDER: PRJNA147265 | ENA |

REPOSITORIES: ENA

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Single-tube linear DNA amplification (LinDA) for robust ChIP-seq.

Shankaranarayanan Pattabhiraman P   Mendoza-Parra Marco-Antonio MA   Walia Mannu M   Wang Li L   Li Ning N   Trindade Luisa M LM   Gronemeyer Hinrich H  

Nature methods 20110605 7


Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cance  ...[more]

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