Dataset Information


Homo sapiens

ABSTRACT: Screening for Epigenetically Masked Genes in Colorectal Cancer using 5-aza-2’-deoxycytidine treatment, Microarray and Gene Expression Profile

ORGANISM(S): Homo sapiens  



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Screening for epigenetically masked genes in colorectal cancer Using 5-Aza-2'-deoxycytidine, microarray and gene expression profile.

Khamas Ahmed A   Ishikawa Toshiaki T   Shimokawa Kazuro K   Mogushi Kaoru K   Iida Satoru S   Ishiguro Megumi M   Mizushima Hiroshi H   Tanaka Hiroshi H   Uetake Hiroyuki H   Sugihara Kenichi K  

Cancer genomics & proteomics 20120301 2

AIM: Unearthing of silenced genes in colorectal cancer (CRC). MATERIALS AND METHODS: Oligonucleotide microarray was used in order to find changes in gene expression in five CRC cell lines before and after 5-aza-2'-Deoxycitidine treatment. Up-regulated genes were integrated with expression profile of matched colorectal tissue samples. Methylation-specific polymerase chain reaction and Real-time quantitative reverse transcription polymerase chain reaction were used to further analyze candidates us  ...[more]

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