Sus scrofa
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ABSTRACT:
PROVIDER: PRJNA147411 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:A mRNA expression study has been performed 20-25 minutes postmortem obtained samples from Longissimus dorsi muscle of 59 Duroc x LD/LW pigs to search for gene sequences related to meat quality (pH24, pH45, “Lab” colour coordinates, curing yield and exudation at three different times) or to meat composition (intramuscular fat, content of several fatty acid (C16:0, C18:0, C18:1 and C18:2), ratio of saturated, monounsaturated and polyunsaturated fatty acids, and protein and humidity contents) traits in order to find targets for selection. Gene ontology analysis, biological pathways and gene networks studies all show, that many more differentially expressed genes (506 vs 279) are related to meat quality (Group P, or perimortem characters) than to meat composition traits (Group L, or whole life traits). The difference between the number of GO terms annotated, biological pathways and gene networks in groups P and L is notable due to the differences in the complexity of the “generation process” of P-traits and the involvement of other tissues or organs in the generation of variability of L-traits. Also, interactions between a list of differentially expressed genes were found in ECM-receptor interaction, TGF-beta signaling pathway, fatty acid elongation in mitochondria and adipocytokine signalling pathway indicating that a substantial fraction of the gene networks could be associated with interactions between differential expressed genes related to traits under study. A high number of the most overexpressed genes are related to muscle development and functionality and repair mechanisms; they could be good candidates for breeding programs whose main goal is to enhance meat quality. 59 Longissimus Dorsi samples from 59 LD/LW x Duroc cross. A total of 33 traits on composition, conformation and meat quality and compositional traits were recorded in the left Longissimus dorsi muscle.
2011-09-19 | E-GEOD-32179 | biostudies-arrayexpress
Project description:Expression data from longissimus dorsi muscle of Shaziling pig at 25-days old and longissimus dorsi musle of Yorkshire pig at 25-days old
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Project description:Comparison among longissimus dorsi muscle of 3 pig breeds, among developmental phases of Tibetan Pig
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Project description:To further characterize genomic regions previously identified which are linked with meat quality traits like drip loss, PH, conductivity and etc., we employed a customary regional tiling array experiment for two pig populations. Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Hybridization were made in batches, 2 to 3 biological replicates for each sample. DuPi and PiF1 are code names for the two experimental populations. Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Hybridization were made in batches, 2 to 3 biological replicates for each sample.
2014-04-01 | E-GEOD-50846 | biostudies-arrayexpress
Project description:To further characterize genomic regions previously identified which are linked with meat quality traits like drip loss, PH, conductivity and etc., we employed a customary regional tiling array experiment for two pig populations. Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Hybridization were made in batches, 2 to 3 biological replicates for each sample. DuPi and PiF1 are code names for the two experimental populations. Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Hybridization were made in batches, 2 to 3 biological replicates for each sample.
2014-04-01 | E-GEOD-52383 | biostudies-arrayexpress
Project description:This study was performed to determine the effects of dietary fat sources, i.e., beef tallow, soybean oil, olive oil and coconut oil (each 3% in feed), on the growth performance, meat quality and gene expression in growing-finishing pigs. The results of this study indicate that the type of dietary fat affects fatty acid composition and insulin signaling-related gene expression in the longissimus dorsi muscle of pigs. Effects of dietary fat types on meat quantity, meat quality and gene expression in pig.
2012-05-22 | E-GEOD-38146 | biostudies-arrayexpress
Project description:Comparison between longissimus dorsi muscle tissue of Fat and Lean Type Pig, among developmental phases
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Project description:The objective of this study was to identify key genes associated with porcine muscle growth and adipose metabolism which different expression in porcine longissimus dorsi muscle tissue among Tibetan Pig, Taihu Pig and Landrace(Month 2), among developmental phases of Tibetan Pig (Month 2,4,6,8). The gene expression analyses will increase understanding of impact factors of pork quality by identifying key genes and pathways controlling longissimus dorsi muscle development. Relative real-time RT-PCR was used to confirm differential expression of 5 different expression genes(PPARGC1A, RYR1, IGF2, IGF1R and IGFBP5) which normalized by 3 housekeep genes(ACTB, TBP and TOP2B). Keywords: breed comparison and time course
2008-05-15 | GSE7858 | GEO
Project description:The objective of this study was to identify key genes associated with porcine muscle growth and adipose metabolism which different expression in porcine longissimus dorsi muscle tissue between Fat Type Pig(Taihu pig) and Lean Type Pig (Landrace), among developmental phases(Month 0,1,2,3,4,5). The gene expression analyses will increase understanding of impact factors of pork quality by identifying key genes and pathways controlling longissimus dorsi muscle development. Relative real-time RT-PCR was used to confirm differential expression of 5 different expression genes(PPARGC1A, RYR1, IGF2, IGF1R and IGFBP5) which normalized by 3 housekeep genes(ACTB, TBP and TOP2B). Keywords: time course and breed comparison
2008-12-16 | GSE7857 | GEO
Project description:We performed a comparative genome-wide methylation analysis of longissimus dorsi muscles between the Japanese Black (Wagyu) and Chinese Red Steppes cattle, which exhibit significant differences in meat quality traits. This will allow us to better understand the correlation between DNA methylation variants and meat quality traits.
2017-08-03 | GSE98818 | GEO