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Hordeum vulgare

ABSTRACT: Transcription profiling of barley plants containing variants of Mla1 and Mla6 powdery mildew resistance genes

OTHER RELATED OMICS DATASETS IN: E-TABM-142

PROVIDER: PRJNA149125 | ENA |

REPOSITORIES: ENA

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Hordeum vulgare

Project description:Comparison of wild-type and cell death mutant of barley plants containing Mla6 powdery mildew resistance gene

| PRJNA111921 | ENA

LC-MSMS of Hordeum vulgare epidermis containing haustoria from Blumeria graminis f.sp. hordei

Project description:Blumeria graminis f.sp. hordei is an obligate biotrohic fungal pathogen causing powdery mildew in barley. As for other biotrophic fungi, haustorial structures are at the centre of the biotrophic interaction and molecular exchanges, delivering fungal effectors or virulence factors, and taking nutrient from the host. Haustoria are originiated by the fungus, following successful penetration of the initial penetration peg through the plant cell call. Haustorial structures mainly of fungal origin, but they are surrounding by a plant component, the extrauhaustorial membrane and matrix (EHM and EHMx) forming the extrahuastorial complex (EHMc). The plant protein make-up of the plant extrahaustorial components remained unexplored, and this is a first study trying to describe plant proteome associated with haustoria using samples enriched for these structures. Therefore, proteomes of haustoria enriched samples from the epidermis of barley leaves infected with Blumeria graminins f.sp. hordei, the causing agent of barley powdery mildew, were compared to infected epidermis and un-infected epidermis to identify haustoria associated plant proteins. Haustoria were enriched from infected epidermis by digesting epidermal cell walls with cell wall degrading enzymes prior to enrichment for haustorial structures. Proteins identified in these samples were compared to infected and uninfected epidermis samples using a non-targeted label free semi-quantitation method.

2019-11-01 | PXD012684 | Pride

Transcription profiling of barley plants containing variants of Mla1 and Mla6 powdery mildew resistance genes

Project description:A split-split-plot design with 144 experimental units (3 replications x 4 genotypes x 6 time points x 2 treatment types) was used to profile barley plants containing variants of Mla1 and Mla6 powdery mildew resistance genes in response to inoculation with the Blumeria graminis f. sp. hordei (Bgh) isolates 5874 (AvrMla1, AvrMla6). Barley leaves were harvested from inoculated and non-inoculated plants at 6 time points (0,8,16,20,24 and 32 hrs) after Bgh inoculation. ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Rico Caldo. The equivalent experiment is BB10 at PLEXdb.]

2011-11-22 | GSE33392 | GEO

Hordeum vulgare

Project description:Mla-specified Transcriptional Responses in Barley-Powdery Mildew Interactions

| PRJNA149133 | ENA

Transcription profiling of wild type and loss-of-function mutants of Mla1 and Mla6 powdery mildew resistant allele barley plants

Project description:A parallel expression profiling of wild-type and loss-of-function mutants of Mla6 and Mla1 powdery mildew resistance alleles was conducted using Barley1 GeneChip. Barley plants were inoculated with powdery mildew isolate 5874 and first leaves were harvested at 6 time points after pathogen inoculation. This experiment was conducted in split-split-plot experimental design with 3 replications.

2008-11-19 | E-TABM-142 | biostudies-arrayexpress

Transcription profiling of wild type and mutants of Sultan V barley plants

Project description:A large-scale time course expression profiling of wild type (Mla12/Rar1/Rom1) and mutants (mla12-M66, M82 (rar1-1), M100 (rar1-2) and rom1) of barley cultivar Sultan 5 was conducted to understand the molecular mechanisms of delayed powdery mildew resistance. Barley plants were inoculated with powdery mildew pathogen isolate 5874. First leaves of inoculated and non-inoculated plants were harvested at six time points after pathogen inoculation. The experiment was laid out in split-split-plot design with 180 experimental units (3 replications x 2 treatments (inoculated and non-inoculated) x 5 genotypes x 6 time points).

2006-08-11 | E-TABM-82 | biostudies-arrayexpress

A comparative transcriptome and chromatin modification analysis provides a resource for characterizing the regulatory roles of epigenetic modifications in hulless barley resistance response to powdery mildew

Project description:We generated ChIP-Seq data for two barley accessions with different resistance to powdery mildew. These data allowed us to explore the roles of epigenetic modifications in resistance response to powdery mildew at the first time. Our study opens the way for establishing the relationship between epigenetics and disease response in barley, and should inform future functional characterization of the regarding molecular basis. These data should also help researchers to exploit disease response-related genes for breeding application.

2021-11-14 | GSE122539 | GEO

Hordeum vulgare

Project description:Transcript profiling of Bln1 silenced plants (BSMV-VIGS) relative to empty vector and buffer treated controls in barley-powdery mildew interactions

| PRJNA261734 | ENA

Barley CI 16151 and fast-neutron-derived, immune-compromised mutants infected with the powdery mildew fungus (Blumeria graminis f. sp. hordei; isolate 5874)

Project description:Purpose: The powdery mildew fungus, Blumeria graminis, is an obligate biotrophic pathogen of cereals and has significant impact on food security (Dean et al., 2012). B. graminis f. sp. hordei (Bgh) is the causal agent of powdery mildew on barley (Hordeum vulgare L.). We sought to address the temporal regulation of membrane trafficking associated gene expression in barley-powdery mildew interactions. We created an isogenic panel of immune signaling mutants to address three main questions: (i) which Blumeria secreted proteins are differentially regulated in response to different compromised genotypes, (ii) which barley membrane trafficking genes are altered in response to pathogen attack, and (iii) how are these genes interacting across genotypes and infection stages.

2018-05-29 | GSE101304 | GEO

mlo5-mediated resistance responses in barley

Project description:Gene expression of a near-isogenic pair of barley lines differing in absence or presence of the mlo5 resistance gene in response to attack by barley powdery mildew (Bgh). ****[PLEXdb(http://www.plexdb.org) has submitted this series at GEO on behalf of the original contributor, Achim Fischer. The equivalent experiment is BB7 at PLEXdb.]

2011-11-22 | GSE33406 | GEO

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