Using microarrays to investigate differential gene expression in gills from smallmouth bass after various exposures.
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ABSTRACT:
PROVIDER: PRJNA150541 | ENA |
REPOSITORIES: ENA
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Project description:The purpose of this study was to investigate differentially expressed genes in gill and liver tissue from smallmouth bass (Micropterus dolomieu) after exposure to various treatments, compared to controls. The treatments consisted of bacteria, estradiol, and a mixture of bacteria plus estradiol.
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Project description:The purpose of this study was to investigate differentially expressed genes in gill and liver tissue from smallmouth bass (Micropterus dolomieu) after exposure to various treatments, compared to controls. The treatments consisted of bacteria, estradiol, and a mixture of bacteria plus estradiol. Four-condition experiment with biological replicates: 4 control replicates, 4 of each treatment for liver and gill tissue.
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Project description:A novel custom microarray for largemouth bass (Micropterus salmoides) was designed from sequences obtained from a normalized cDNA library using the 454 Life Sciences GS-20 pyrosequencer. The GS-20 yielded in excess of 58 million bases of high-quality sequence. The sequence information was combined with 2,616 reads obtained by traditional suppressive subtractive hybridizations to derive a total of 31,391 unique sequences. Annotation and coding sequences were predicted for these transcripts where possible. 16,350 annotated transcripts were selected as target sequences for the design of the custom largemouth bass oligonucleotide microarray. The microarray was validated by examining the transcriptomic response in male largemouth bass exposed to 17 -oestradiol. Transcriptomic responses were assessed in liver and gonad, and indicated gene expression profiles typical of exposure to oestradiol. The results demonstrate the potential to rapidly create the tools necessary to assess large scale transcriptional responses in non-model species, paving the way for expanded impact of toxicogenomics in ecotoxicology. Keywords: E2 exposure, array validation This experiment tested two organs - liver and gonad from either E2-exposed or control fish, 4 fish (biological replicates) per treament (control and E2).
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