Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Homo sapiens

ABSTRACT: Identification of Proteins Binding Coding and Non-coding Human RNAs using Protein Microarrays

OTHER RELATED OMICS DATASETS IN: GSE40123

PROVIDER: PRJNA151039 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Identification of Proteins Binding Coding and Non-coding Human RNAs using Protein Microarrays

2012-10-10 | GSE34794 | GEO

Identification of Proteins Binding Coding and Non-coding Human RNAs using Protein Microarrays

Project description:We describe a refined approach to identify new human RNA-protein interactions. In vitro transcribed labeled RNA is bound to ~9,400 human recombinant proteins spotted on protein microarrays. This approach identified 137 RNA-protein interactions for 10 human coding and non-coding RNAs, including an interaction between Staufen 1 protein and TP53 mRNA that promoted the latter’s stability. RNA hybridization to protein microarrays allows rapid identification of human RNA-protein interactions on a large scale. Sense and antisense strands for 10 RNA transcripts representing protein coding RNAs TP53, HRAS, MYC, BCL2 and non-coding sequences PWRN1, SOX2OT, OCC1, IGF2RNC, lncRBM26 and DLEU1 were in vitro transcribed, labeled with Cy5 and independently hybridized on human protein microarrays. The labeling process was optimized in order to achieve ~ 3 pmol dye per every microgram RNA with average efficacy of 1 dye molecule for approximately every 850 bp RNA to minimally influence RNA native structure and at the same time yield in signal intensities that were readily visualized.

2012-10-10 | E-GEOD-34794 | biostudies-arrayexpress

Mus musculus

Project description:Sequencing of the non-ribosomal transcriptome allows the simultaneous identification of protein-coding and non-protein-coding RNAs

| PRJNA127845 | ENA

Homo sapiens

Project description:Identification of expressed and conserved human non-coding RNAs

| PRJNA193501 | ENA

Identification of NLRP3 inflammasome binding non-coding RNAs in activated iBMDMs

Project description:Identification of NLRP3 inflammasome binding non-coding RNAs in activated iBMDMs

| PRJNA486535 | ENA

Relevance study and prognostic analysis for intestinal flora disorders affecting non-coding RNA networks in patients with advanced lung cancer

Project description:Interventions: Case series:Nil Primary outcome(s): intestinal microecological disorders;blood non-coding RNAs and immune status Study Design: Randomized parallel controlled trial

| 2681498 | ecrin-mdr-crc

Project description:Identification of long non-coding RNAs using long-read cDNA sequencing

| PRJNA765684 | ENA

Bacterial non-coding RNAs excised from within protein-coding transcripts

Project description:Bacterial non-coding RNAs excised from within protein-coding transcripts

| PRJEB27518 | ENA

Homo sapiens

Project description:Finding new proteins encoded by long non-coding RNAs in human cells

| PRJNA316125 | ENA

Danio rerio

Project description:Identification of small non-coding RNAs in zebrafish

| PRJNA277780 | ENA