Project description:Gene expression profiling of elutriated monocytes cultured for 48 hours in RPMI-1640, 10% AB plamsa and one of the following: GMCSF (56IU/ml), IL-4 (2000 IU/ml), 15 kDa Granulysin (10 nM) Sources tested: monocytes form 3 donors tested at time 0, 4 hours, 12 hours, 24 hours, 48 hours
Project description:This study set out to identify global changes in gene expression in human embryonic kidney (HEK) cells stably transfected with Toll-like receptor 2 (TLR2) over a 48 hour time-course, following stimulation with 10 μg/ml lipopolysaccharide (LPS) from the gastric pathogen H. pylori. Microarray analysis was used to compare changes in gene expression between cells treated with 10 μg/ml H. pylori LPS and untreated cells at the same time point.
Project description:In this project, we report the bacterial proteome expression profile change for different time points when culturing in rapeseed cake substrate (RCS). Bacillus cereus tsu1 was cultured in RCS (25g/L) for 12 hours, 24 hours and 48 hours. At each time point, cells were stained with Sudan Black to monitor PHB accumulation. Bacterial cells were loaded with PHB after 12-hour culture and significant degradation of PHB was observed after 48-hour culture. Bacterial proteome profile changes were identified using tandem mass tags mass spectrometry (TMT-MS)-based quantitative proteomics analysis. From TMT proteomics analysis, 3,237 proteins were detected and quantified from the bacterial pellet protein extraction, from which proteins related with PHB biosynthesis and degradation were identified. Quantitative analysis revealed that 146 (between 12h and 24h samples), and 158 (between 12h and 48h) proteins showed significant differences. Several STRING protein interaction networks were developed for significantly changed protein related with sporulation and anaerobic respiration.
Project description:Gene expression profiling of elutriated monocytes cultured for 48 hours in RPMI-1640, 10% AB plamsa and one of the following: GMCSF (56IU/ml), IL-4 (2000 IU/ml), 15 kDa Granulysin (10 nM) Sources tested: monocytes form 3 donors tested at time 0, 4 hours, 12 hours, 24 hours, 48 hours 5 time points were compared: day 0 (cells before start the culture), 4, 12, 24, 48 hours
Project description:Analysis of alternative activation of macrophages at gene expression level. The study forms part of a wider study where we compare the effects of IL-4 in different human and mouse macrophages. Our results support the notion that in vitro culture conditions greatly affect the macrophage response to IL-4. Total RNA obtained from human monocyte derived macrophages upon exposure to 10 ng/ml of IL-4 for 48 hours. Monocytes were induced to mature into macrophages using X Vivo 10 medium supplemented with 1% autologous serum.
Project description:To determine metabolite concentrations and differences at the 48 hour time point for WT, ClpC mutant, srrAB mutant, and ClpC:srrAB double mutant
Project description:Analysis of gene expression by RNA-seq upon siRNA mediated knockdown of scaffold attachment factor A (SAF-A) versus control siRNA in RPE1 cells at 24 hour and 48 hour time points post transfection reveals SAF-A loss does not impact on gene transcription
Project description:To uncover the variation in protein abundances during 48 hours under light and dark cycles (12:12-hours), we used quantitative proteomics, with TMT 6-plex isobaric labeling. We queried the S. elongatus proteome at ten different time points spanning a single 24 hour period, leading to 20 time points over the full 48 hour period.
Project description:To identify patterns of change in gene expression that correlated with drug treatment (saline control, ADH-1 alone, melphalan alone and ADH-1 plus melphalan) in our animal model of regional therapy for extremity melanoma we evaluated gene expression using microarray genechips and a Pearson correlation analysis. Genes were ranked according to the degree to which expression correlated with systemic ADH-1 treatment alone or in combination with regional melphalan. Tissue samples were harvested at 4, 24 and 48 hours after treatment with melphalan. Specific cellular pathways associated with drug treatment were identified using gene set enrichment analysis (GSEA). Experiment Overall Design: Sample size was 2 rats for each drug treatment group at both the 4 and 24 hour time points. At the 48 hour time point sample size was 1 rat for each drug treatment group. ADH-1 treatment preceded melphalan by 1 hour. ADH-1 treatment was repeated at 24 and 48 hrs. Tissue was harvested for RNA analysis at 4, 24 and 48 hours after completion of isolated limb infusion with melphalan (and 1 hour after an ADH-1 dose).
Project description:PPARα act as the master of lipid metabolism in liver, however, the changes of its target genes after PHx and the effects of PPARα on regenerative genes were unknown. At 12 to 24 hours after PHx, the mice develope marked steatosis, therefore the time point of 12 hour after PHx was choosen to perform microarray analysis. We used microarray to detail the gene expression of WT (Pparafl/fl) mice and hepatocyte-specific PPARα disruption (Ppara△Hep) mice liver tissue at 12 hour after PHx or Sham operation
