Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA151637 | ENA |
REPOSITORIES: ENA
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Project description:High-throughput sequencing of HMW RNAs (4-10 kb) from Drosophila Dnmt2 mutants during heat shock recovery
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Project description:Dnmt2 genes are highly conserved tRNA methyltransferases with biological roles in cellular stress responses. Dnmt2 has recently been implicated in transposon silencing in Drosophila but the exact molecular mechanisms are unclear. Adult Dnmt2 mutants were heat shocked and RNA sequencing was performed on visible high-molecular weight RNAs to determine the identity of up-regulated transposons. Dnmt2 mutants accumulated almost all families of transposons after heat shock, indicating a general mis-regulation of transposon silencing in Dnmt2 mutants during the stress response.
2014-12-01 | GSE40432 | GEO
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster S2 and KC cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt
2008-04-30 | E-GEOD-10794 | biostudies-arrayexpress
Project description:Dnmt2 and NSun2 are (cytosine-5) RNA methyltransferases. Using CRISPR/Cas9 we created null mutations in Dnmt2 and NSun2 genes in Drosophila melanogaster. We also ectopically expressed a wild type and catalytically inactive Dnmt2 form in the Dnmt2 mutant background. RNA bisulfite sequencing was used to follow RNA methylation at partical tRNA substrates.
2018-01-16 | GSE109133 | GEO
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Small RNAs were sequenced from D. melanogaster heads (male and female), body (male and female), S2 and Kc cells and different stages of embryo. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt
2008-06-01 | E-GEOD-11624 | biostudies-arrayexpress
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: High-throughput solexa sequencing Small RNAs were sequenced from D. melanogaster female head. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt Quality scores in the supplementary file for GSM240749 are undefined. Quality of the bases assessed by (1) identifying for the sequenced linker, which is a known sequence, and (2) mapping the clipped sequence to the genome and taking only perfect hits.
2008-04-22 | E-GEOD-10790 | biostudies-arrayexpress
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from S2 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster S2 cells obtained from Gerald Rubin's lab. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.
2009-01-22 | E-GEOD-14488 | biostudies-arrayexpress
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from imaginal disc / brain, female body and male body. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: small RNA discovery and profiling For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster adult bodies. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.
2009-04-30 | E-GEOD-15898 | biostudies-arrayexpress
Project description:High-throughput sequencing of Drosophila melanogaster small RNAs from Kc167 cells. total RNA, ~18-26nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Keywords: small RNA discovery and profiling For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Small RNAs were sequenced from D. melanogaster Kc167 cells. Raw sequences were clipped by 3' linker sequences recognition, and select clipped sequences longer than 18 nt.
2009-04-30 | E-GEOD-15897 | biostudies-arrayexpress
Project description:High-throughput sequencing of small RNAs from Drosophila
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