Project description:Geographical distinct virulent Babesia bovis strains have similar gene expression changes as they go through attenuation. Pair end RNA-sequencing reads on three biological replicate sample pairs of virulent parent and attenuated derivative Babesia bovis strain isolated in Argentina.
Project description:Babesia parasites transition between a mammalian host, where they cause babesiosis, and the tick vector that transmits them. This transition provides an environmental signal resulting in altered gene expression allowing the completion of the parasite’s life cycle. A comparison of the different life stages that occur within mammalian and tick hosts can provide insight into the adaptation of Babesia to these different environments. In this study, we used RNA-Seq to compare gene expression between Babesia bovis blood stages and tick derived kinetes.
Project description:Babesia bovis exposed tissues: Three tissues were looked at. 1. Adult Female Gut 2. Adult Ovary 3. Larvae. In all there are 24 measurements for feature (EST), and 4 measurements per treatment for each of the 6 treatment groups. The 6 treatment groups are: Gut infected (GI), Gut control (GC), and similarly for Ovary and Larval: OI, OC, LI, LC. There are 12 chips, each with a spot replicate. **Note: contact person: Felix D. Guerrero email: felix.guerrero@ars.usda.gov Keywords: Rhipicephalus (Boophilus) microplus, Babesia, microarrays.
Project description:To understand Babesia gene regulation during tick and mammalian host infection, we performed high throughput RNA-sequencing using samples collected from calves and Rhipicephalus microplus ticks infected with Babesia bigemina. We evaluated gene expression differences between B. bigemina kinetes and blood-stage parasites
