Project description:Purpose: This study identifies the top transcripts expressed in mature erythrocytes and the top transcripts expressed in the polysome fractions of mature erythrocyte lysate Methods: RNA from total erythrocyte lysate and polysome fractions was isolated and purified using GeneJet RNA purification column (Thermo Fisher Scientific). The RNA library was prepared using NEBNext ULTRA II RNA library preparation kit for Illumina. The libraries were sequenced using Illumina Seq. Results: The transcriptome analysis revealed the abundance of mRNAs encoding globins genes, long non-coding RNAs, and micro RNAs. Deciphering the role and functions of these non-coding RNAs in these specialised cells could enable us to understand their biology in greater detail

2019-03-22 | GSE128648 | GEO

Homo sapiens

Project description:Transcriptomic analysis reveals novel long non-coding RNAs critical for vertebrate development [RNA-Seq]

| PRJNA238180 | ENA

Comprehensive RNA-Seq profiling in PBMCs of ALS patients and healthy controls

Project description:Coding and long non-coding RNA metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we performed Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from Sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1, C9Orf72 and VCP genes) and healthy controls. Our aim is the whole-transcriptome characterization of PBMCs content, both in terms of coding and non coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Out dataset is a starting point for the omni-comprehensive analysis of coding and long non coding RNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS.

2018-07-04 | GSE115259 | GEO