Drosophila melanogaster
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA156227 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Differential Expression in UpSET mutant ovaries
| PRJNA156223 | ENA
Project description:AGO1 immunoprecipitated small RNAs from D. melanogaster wildtype and mutant ovaries
| PRJNA268781 | ENA
Project description:Developmental gene expression results from the orchestrated interplay between genetic and epigenetic mechanisms. Here, we describe upSET, a transcriptional regulator encoding a SET domaincontaining protein recruited to active and inducible genes in Drosophila. However, unlike other Drosophila SET proteins associated with gene transcription, UpSET is part of an Rpd3/Sin3-containing complex that restricts chromatin accessibility and histone acetylation to promoter regions. In the absence of UpSET, active chromatin marks and chromatin accessibility increase and spread to genic and flanking regions due to destabilization of the histone deacetylase complex. Consistent with this, transcriptional noise increases, as manifest by activation of repetitive elements and off-target genes. Interestingly, upSET mutant flies are female sterile due to upregulation of key components of Notch signaling during oogenesis. Thus UpSET defines a class of metazoan transcriptional regulators required to fine tune transcription by preventing the spread of active chromatin. Total mRNA from wildtype and epic mutant ovaries was extracted, labeled and hyb on a custom tiling arrays. Two biological replicates were performed.
2012-12-07 | E-GEOD-34719 | biostudies-arrayexpress
Project description:Gene expression analyses in otefin mutant Drosophila ovaries
| PRJNA376599 | ENA
Project description:Expression data from bam and setdb1 mutant ovaries
| PRJNA312580 | ENA
Project description:Gene expression analysis of spargel mutant versus genetically matched wildtype control
| PRJNA112211 | ENA
Project description:Drosophila Pph13 mutant vs. wildtype and Orthodenticle, Pph13 double mutant vs. wildtype
| PRJNA128343 | ENA
Project description:H3K27me3 profiles using Cleavage under targets and Release using nuclease (Cut&Run) in control and KD Drosophila melanogaster ovaries. We examined the impact on chromatin profiles in Drosophila melanogaster ovaries in which the lid, the Sin3a, the Snr1 or the mod(mdg4) gene have been selectively knocked down by tissue-specific shRNA expression. We additionally explored H3K27me3 and H3K9me3 in control and dhd mutant ovaries either carrying or not a transgene.
2021-12-10 | GSE174250 | GEO
Project description:Expression data from wild type and ttk^twk mutant whole ovaries
| PRJNA183069 | ENA
Project description:Chromatin plays a critical role in faithful implementation of gene expression programs. Different post-translational modifications of histone proteins reflect the underlying state of gene activity, and many chromatin proteins write, erase, bind, or are repelled by these histone marks. One such protein is UpSET, the Drosophila homolog of yeast Set3 and mammalian KMT2E (MLL5). Here we show that UpSET is necessary for the proper balance between active and repressed states. Using CRISPR/Cas-9 editing, we generated S2 cells which are mutant for upSET. We found that loss of UpSET is tolerated in S2 cells, but that heterochromatin is misregulated, as evidenced by a strong decrease in H3K9me2 levels assessed by bulk histone post-translational modification quantification. To test whether this finding was consistent in the whole organism, we deleted the upSET coding sequence using CRISPR/Cas-9, which we found to be lethal in both sexes in flies. We were able to rescue this lethality using a tagged upSET transgene, and found that UpSET protein localizes to transcriptional start sites of active genes throughout the genome. Misregulated heterochromatin is apparent by suppressed position effect variegation of the wm4 allele in heterozygous upSET-deleted flies. We show that this result applies to heterochromatin genes generally using nascent-RNA sequencing in the upSET-mutant S2 lines. Our findings support a critical role for UpSET in maintaining heterochromatin, perhaps by delimiting the active chromatin environment.
2017-03-07 | GSE90703 | GEO