Metarhizium robertsii
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA158211 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Metarhizium robertsii is one of the model fungus species widely used studying insect-fungus interactions. Our previous studies have shown the accumulation of lipid droplets (LDs) play an essential role in generation of cellular turgor pressure to assist fungal penetration of insect cuticles, and autophagy-related proteins are connected with LD biogenesis and cellular accumulation in M. robertsii. However, full proteomes of LDs are unclear in terms of the species of proteins involved in lipid biosynthesis and degradation. We performed experiments by growing the fungi in different nutrient conditions, isolated the LDs and extracted the LD proteins from the cultures after being grown in a nutrient-rich medium (i.e. Sabouraud dextrose broth, SDB), a minimum medium without nitrogen source (MM-N) and MM-N supplemented with oleate. The protein samples were then subject to LC-MS/MS proteome profilings for identifying and postulating the proteins/pathways involved in lipid metabolisms.
2022-03-01 | PXD001552 | Pride
Project description:a high-throughput Illumina/Solexa sequencing was conducted, 478262, 702593, 835394 and 894277 unique sRNAs was discovered from four periods of M. robertsii infection, uninfected (0h), infected for 12h, 24h and 36h. Then, 7, 7, 6 and 7 known pre-miRNAs were obtained, and 33, 58, 54 and 52 candidate novel miRNAs was detected in four periods. Further analysis showed that 24 of those candidate novel miRNAs were matched to other known insect miRNAs, while 36 of those miRNAs lacked sequence homologues of insect organisms.
2022-04-01 | GSE99322 | GEO
Project description:High-throughput sequencing of RNAs isolated by cross-linking immunoprecipitation in Metarhizium robertsii
2019-06-05 | GSE82296 | GEO
Project description:Exopolysaccharide galactosaminogalactan (GAG) is a fungal cell wall component composed of α-1,4 linked galactose, N-acetyl galactosamine and galactosamine, which has been demonstrated in Aspergillus fumigatus in association with fungal adhesion, biofilm formation and virulence. The gene cluster responsible for GAG biosynthesis has only been characterized in Aspergillus fungi. We found that the highly conserved gene cluster for GAG biosynthesis is also present in the insect pathogenic fungi Metarhizium species. Functional investigations in M. robertsii revealed that GAG is only produced on fungal cell wall during fungal germination, filamentation and the formation of the infection structure appressoria. Gene deletions revealed that, relative to the wild-type (WT), the appressorial mucilage production was abolished in the null mutant of M. robertsii. Since multiple enzymes are produced in appressorial mucilages, appressorial samples of the WT and mutant formed on cicada wings were collected and subjected to iTRAQ comparative proteomic analysis. We found that different protein families were up- or down-regulated in the null mutant when compared with the WT.
2021-06-07 | PXD019301 | Pride
Project description:Insect pathogenic fungus Beauveria bassiana in one of the best studied insect biocontrol fungus, which infects insects by cuticle penetration. After breaking the cuticles, the fungus will propagate in insect hemocoel and kill insect hosts. It has also been found that the mycelia of B. bassiana can penetrate plant tissues to reach insect inside plant, e.g. corn borer (Ostrinia furnacalis), but do not cause damage to plants. The mechanism of fungal physiological plasticity is poorly understood. To accompany our genome sequencing work of B. bassiana strain ARSEF 2860, fungal transcriptional responses to different niches were studied using an Illumina RNA_seq technique. To examine fungal response to insect cuticle, conidia were inoculated on locust hind wings for 24 hours before used for RNA extraction. To evaluate fungal adaptation to insect hemocole, the fifth instar larvae of cotton bollworms were injected with spore suspension and fungal cells isolated by centrifugation in a step gradient buffer. To unveil the mechanism of interaction with plants, the fungus was grown in corn root exudates for 24 hours. After RNA sequencing, around three million tags were acquired for each sample and fungal transcriptional profiles were compared. Unveiling gene differential expression patterns when the insect biocontrol fungus Beauveria bassiana grown in insect hemocoel, corn root exudates and on insect cuticles.
2012-07-10 | E-GEOD-32699 | biostudies-arrayexpress
Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by Cfp1, which is evidently required for insect pathogenicity and virulence-related cellular events of Metarhizium robertsii. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of cfp1 disruption and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Metarhizium robertsii. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10681 detected genes. Among those, 605 genes were dysregulated in the absence of cfp1, including 356 down-regulated and 249 up-regulated. Conclusions: Cfp1 lacking any predictable function domain has profound impact on the genomic expression of Metarhizium robertsii.
2022-02-26 | GSE197269 | GEO
Project description: Not available
| S-EPMC5996154 | biostudies-literature
Project description:Genome-wide analysis of DNA methylation Metarhizium robertsii
| PRJNA312369 | ENA
Project description:Mycelium growth and conidiogenesis of Metarhizium robertsii
| PRJNA319820 | ENA
Project description:Metarhizium robertsii Raw sequence reads
| PRJNA357941 | ENA