Project description:Pristionchus pacificus has emerged as a valuable model system for comparative and evolutionary-developmental biology (evo-devo) studies alongside the classic model nematode C. elegans. Previous studies have identified a lack of conservation of genetic networks underlying conserved traits, referred to as developmental systems drift. However, the conservation – or lack thereof – of epigenetic pathways which regulate development have not been investigated. In the manuscript associated with this study, we present an “epigenetic toolkit” for P. pacificus and C. elegans to compare and contrast epigenetic pathways. Assembly of this toolkit was done by identifying orthologous genes, including the “writers” and “erasers” of histone modifications. To complement this evolutionary approach, here we produce a data set of the suite of histone modifications present in P. pacificus.
Project description:Transcriptional profiling of germline-ablated P. pacificus worms exposed to S. marcescens for 8 hours versus germline-ablated P. pacificus exposed to the control E. coli OP50. First goal was to identify genes that are involved in immune response of germline-ablated P. pacificus when exposed to S. marcescens. Second, this was compared to longevity-regulating genes in germline-deficient animals (see series GSE37331).
Project description:Transcriptional profiling of germline-ablated P. pacificus worms exposed to S. marcescens for 8 hours versus germline-ablated P. pacificus exposed to the control E. coli OP50. First goal was to identify genes that are involved in immune response of germline-ablated P. pacificus when exposed to S. marcescens. Second, this was compared to longevity-regulating genes in germline-deficient animals (see series GSE37331). One-condition experiments. Germline ablated P. pacificus worms exposed to S. marcescens for 8 hours versus germline-ablated P. pacificus exposed to the control E. coli OP50. 4 biological replicates for each condition, including 2 dye-swaps.
Project description:Transcriptional profiling of germline-ablated P. pacificus worms versus un-ablated wild-type controls. Food source E. coli OP50 for both conditions. The goal was to identify genes that regulate the enhanced longevity observed in germline-deficient animals. One-condition experiments. Germline ablated P. pacificus versus un-ablated wild-type P. pacificus. Developmental stage = Young adults. Food source = E. coli OP50 for both conditions. 3 biological replicates for each condition, including 2 dye-swaps.
