Project description:Using an integrated systems approach, the expressed proteome of B. diazoefficiens strain 110scp4 was measured under i) normal, oxic growth, and ii) microoxic growth condtions. This included, as a first step, the sequencing and de novo assembly of the genome of this widely used rhizobial model strain, which turned out to harbor several deletions and insertions compared to the B. diazoefficiens USDA 110 NCBI reference genome. With this optimal basis in hand, a shotgun proteomics approach relying on a slightly adapated FASP protocol was carried out, allowing to identify 2900 (oxia) and 2826 (microoxia) proteins, respectively, thereby largely expanding the proteome known to be expressed under microoxic conditions.

2019-05-30 | PXD012491 | Pride

Bradyrhizobium diazoefficiens USDA 110

Project description:Pleiotropic effects of PhaR involved in poly-beta-hydroxybutyrate accumulation in Bradyrhizobium diazoefficiens USDA 110

| PRJDB5605 | ENA

Bradyrhizobium diazoefficiens

Project description:Bradyrhizobium diazoefficiens overview

| PRJNA211929 | ENA

Bradyrhizobium diazoefficiens

Project description:Bradyrhizobium diazoefficiens project

| PRJDB9719 | ENA

Bradyrhizobium diazoefficiens

Project description:Bradyrhizobium diazoefficiens Genome sequencing and assembly