Project description:What methylation changes are occurring in different parts of early maturation stage seed largely remains unknown. To uncover the possible role of DNA methylation in different parts of early maturation stage seed, we characterized the methylome of seed coats,cotyledons, and the embryonic seed axis using Illumina sequencing. seed coats, cotyledon, and axis

2012-05-11 | E-GEOD-37895 | biostudies-arrayexpress

Metabolic and Transcriptional Reprogramming in Developing Soybean (Glycine max) Embryos

Project description:Soybean (Glycine max) seeds are an important source of seed storage compounds, including protein, oil, and sugar used for food, feed, chemical, and biofuel production. We assessed detailed temporal transcriptional and metabolic changes in developing soybean embryos to gain a systems biology view of developmental and metabolic changes and to identify potential targets for metabolic engineering. Two major developmental and metabolic transitions were captured enabling identification of potential metabolic engineering targets specific to seed filling and to desiccation. The first transition involved a switch between different types of metabolism in dividing and elongating cells. The second transition involved the onset of maturation and desiccation tolerance during seed filling and a switch from photoheterotrophic to heterotrophic metabolism. Clustering analyses of metabolite and transcript data revealed clusters of functionally related metabolites and transcripts active in these different developmental and metabolic programs. The gene clusters provide a resource to generate predictions about the associations and interactions of unknown regulators with their targets based on guilt-by-association relationships. The inferred regulators also represent potential targets for future metabolic engineering of relevant pathways and steps in central carbon and nitrogen metabolism in soybean embryos and drought and desiccation tolerance in plants.

2013-12-13 | GSE46153 | GEO

Expression profiling of Glycine max seed maturation by high throughput sequencing

Project description:A transcriptome analysis of soybean seeds harvested at different developing stages (between stage 7.1 and stage 9) was carried out to understand the molecular events occuring during the acquisition of seed longevity during maturation.

2017-05-01 | GSE98199 | GEO

Lignin deposits in xylem vessels of pedicel regulate water transport during seed maturation in soybean (Glycine max L. Merr.)

Project description:Seed desiccation during maturation is important process for seed post-maturation behavior and harvest. However, the desiccation mechanism in soybean seed maturation is hardly known. In this study, water content in seed, pod and pedicel decreased faster than that in peduncle and stem. Therefore, we focus on the pedicel during seed maturation. By morphological analysis, the deposits in xylem vessels were confirmed in pedicel at 61 day after flowering (DAF), when there are not the deposits in peduncle. It was clarified by microarray analysis that lignin biosynthesis related genes expressed in pedicel at 61 DAF. Indeed, GmPAL, Gm4CL, GmC3H and GmCAD, which are lignin biosynthesis related genes, increased in pedicel during seed maturation. Furthermore, lignin content in pedicel also increased toward at 61 DAF and accumulated in the xylem vessels. These results suggested that lignin deposits into xylem vessels in pedicel cause the soybean seed desiccation during seed maturation.

2021-04-01 | GSE136771 | GEO

Glycine max

Project description:Expression data from soybean seed compartments with embryos at the early maturation stage

| PRJNA100981 | ENA