Komagataella phaffii GS115
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA169169 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Komagataella phaffii GS115 Transcriptome or Gene expression
| PRJNA705382 | ENA
Project description:Komagataella phaffii GS115 Raw sequence reads
| PRJNA414726 | ENA
Project description:Komagataella phaffii GS115 Genome sequencing and assembly
| PRJNA304976 | ENA
Project description:Ribosome profiling in Komagataella phaffii
| PRJNA669501 | ENA
Project description:Mechanism of heterologous protein expression in Komagataella phaffii based on transcriptome
| PRJDB5592 | ENA
Project description:Transcriptome analysis of mig1Δmig2Δ mutant of Pichia pastoris GS115
| PRJNA355965 | ENA
Project description:Komagataella phaffii strain:pPIC3.5K-gfp Transcriptome or Gene expression
| PRJNA658827 | ENA
Project description:The protein production host Komagataella phaffii has possess the ability to differentiate into pseudohyphal form when cultivated at slow growth rates (µ=0.05 h-1) in glucose-limited chemostats. In this study, we investigated the K. phaffii FLO gene family in the context of pseudohyphae formation. Transcriptional analysis helped us identify 3 possible responsible genes, FLO11, FLO400 and FLO5-1, all of which are under control of Flo8, a transcription factor whose disruption prevents pseudohyphae formation. Knocking out FLO11 revealed that this is not the sole protein responsible for this phenotype. Strikingly, the expression of FLO400 and FLO5-1 was negatively correlated with pseudohyphae formation, and shown to be under epigenetic control by FAIRE-Seq analysis. Knock outs of these two genes completely inhibited the appearance of pseudohyphal cells and prevented the expression of FLO11. Even though the mechanism is unclear at present, we propose that in K. phaffii Flo400 and/or Flo5-1 act as upstream signals that lead to the induction of FLO11 expression upon severe glucose limitation in chemostats at slow growth rate, and that the expression of FLO400 and FLO5-1 is controlled by epigenetic silencing, which acts independently from the general activation of FLO gene expression by the transcriptional regulator Flo8.
2020-08-19 | GSE131290 | GEO
Project description:The protein production host Komagataella phaffii has possess the ability to differentiate into pseudohyphal form when cultivated at slow growth rates (µ=0.05 h-1) in glucose-limited chemostats. In this study, we investigated the K. phaffii FLO gene family in the context of pseudohyphae formation. Transcriptional analysis helped us identify 3 possible responsible genes, FLO11, FLO400 and FLO5-1, all of which are under control of Flo8, a transcription factor whose disruption prevents pseudohyphae formation. Knocking out FLO11 revealed that this is not the sole protein responsible for this phenotype. Strikingly, the expression of FLO400 and FLO5-1 was negatively correlated with pseudohyphae formation, and shown to be under epigenetic control by FAIRE-Seq analysis. Knock outs of these two genes completely inhibited the appearance of pseudohyphal cells and prevented the expression of FLO11. Even though the mechanism is unclear at present, we propose that in K. phaffii Flo400 and/or Flo5-1 act as upstream signals that lead to the induction of FLO11 expression upon severe glucose limitation in chemostats at slow growth rate, and that the expression of FLO400 and FLO5-1 is controlled by epigenetic silencing, which acts independently from the general activation of FLO gene expression by the transcriptional regulator Flo8.
2020-08-19 | GSE131106 | GEO