Project description:Investigation of the contribution of Complex I to respiratory energy-coupling in glucose-grown cultures of the Crabtree-negative yeast Ogataea parapolymorpha
Project description:We analyze the transcriptome of Ogataea parapolymorpha wild-type and met4 mutant under sulfur limited condition to investigate the function of Met4 protein in this yeast in the absence of any sulfur source. By sequencing the mRNAs of the wild-type and mutant grown in sulfur-limited B minimal medium with or without cysteine as a sole sulfur source, we find the role of OpMet4p as a master regulator for cell homeostasis under sulfur limitation.
Project description:The methylotrophic, thermotolerant yeast Ogataea parapolymorpha (formerly Hansenula polymorpha) is an industrially relevant production host and exhibits a respiratory metabolism in the presence of oxygen. It possesses a branched respiratory chain with multiple entry points for NADH-derived electrons that differ in complexity and degree of energy conservation: proton-translocating respiratory Complex I and three putative alternative NADH dehydrogenases. To investigate the physiological importance of Complex I, wild type O. parapolymorpha and a Complex I-disrupted mutant were cultured in glucose-grown bioreactor experiments in batch, chemostat and retentostat cultivations which allowed quantitative characterization of the strains over a wide range of growth rates in the presence and absence of excess substrate.
