Homo sapiens
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA171152 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Breaking up prolonged periods of time spent sitting has a range of beneficial impacts on cardiometabolic risk biomarkers. The molecular mechanisms include regulation of skeletal muscle gene and protein expression controlling metabolic, inflammatory and cell development pathways. An active communication network exists between adipose and muscle tissue, but the effect of active breaks in prolonged sitting on adipose tissue have yet to be investigated. This study characterised the acute transcriptional events induced in adipose tissue by regular active breaks during prolonged sitting. In a subset of 8 overweight/obese adults participating in an acute randomised three-intervention crossover trial, subcutaneous adipose tissue biopsies were obtained after each condition. The three experimental conditions were conducted in the postprandial state and included: i) prolonged uninterrupted sitting; or prolonged sitting interrupted with 2-minute bouts of ii) light- or iii) moderate-intensity treadmill walking every 20 minutes. Microarrays identified 36 differentially expressed genes between the three conditions (fold change≥0.5 in either direction; p<0.05). Pathway analysis indicated that breaking up of prolonged sitting led to differential regulation of adipose tissue metabolic networks and inflammatory pathways, increased insulin signalling, increased adipocyte turnover, and facilitated cross-talk between adipose tissue and other organs. This study provides insight into the adipose tissue regulatory systems and transcriptional processes that contribute to the physiological benefits of interrupting prolonged sitting.
2018-06-13 | GSE115645 | GEO
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression.
2011-02-19 | GSE20463 | GEO
Project description:Breaking up prolonged sitting has been shown to be beneficially associated with cardio-metabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment cross-over trial in an acute setting (5 hours): seated position with no activity, seated with 2-minute bouts of light- or moderate-intensity treadmill walking every 20 minutes. Vastus lateralis biopsies were obtained in 8 of the 19 participants after each treatment and gene expression examined using microarrays validated with real-time qPCR. Total RNA was extracted from vastus lateralis at the 3 different experimental condition
2012-07-24 | E-GEOD-39576 | biostudies-arrayexpress
Project description:Breaking up prolonged sitting has been shown to be beneficially associated with cardio-metabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment cross-over trial in an acute setting (5 hours): seated position with no activity, seated with 2-minute bouts of light- or moderate-intensity treadmill walking every 20 minutes. Vastus lateralis biopsies were obtained in 8 of the 19 participants after each treatment and gene expression examined using microarrays validated with real-time qPCR.
2012-07-25 | GSE39576 | GEO
Project description:Breaking up prolonged sitting has been shown to be beneficially associated with cardio-metabolic risk markers in both observational and intervention studies. We aimed to define the acute transcriptional events induced in skeletal muscle by breaks in sedentary time. Overweight/obese adults participated in a randomized three-period, three-treatment cross-over trial in an acute setting (5 hours): seated position with no activity, seated with 2-minute bouts of light- or moderate-intensity treadmill walking every 20 minutes. Vastus lateralis biopsies were obtained in 8 of the 19 participants after each treatment and gene expression examined using microarrays validated with real-time qPCR.
2012-07-25 | GSE39576 | GEO
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.
2011-08-25 | GSE23936 | GEO
Project description:Gene expression profiling of immortalized human mesenchymal stem cells with hTERT/E6/E7 transfected MSCs. hTERT may change gene expression in MSCs. Goal was to determine the gene expressions of immortalized MSCs.
2010-09-25 | GSE24338 | GEO
Project description:Transcriptional profiling of human mesenchymal stem cells comparing normoxic MSCs cells with hypoxic MSCs cells. Hypoxia may inhibit senescence of MSCs during expansion. Goal was to determine the effects of hypoxia on global MSCs gene expression. Two-condition experiment, Normoxic MSCs vs. Hypoxic MSCs.
2011-02-19 | E-GEOD-20463 | biostudies-arrayexpress
Project description:Effects of mixed exercise training on gene expression in human skeletal muscle
| PRJNA232469 | ENA