Project description:We report the identification of small RNAs in undifferentiated (d0) and differentiating (d4) mouse embryonic stem (ES) cells using high-throughput sequencing. The goal of this study was to identify small RNAs involved in X-chromosome inactivation (XCI). We have identified a subset of small RNAs that are generated from transposon sequences and map to the X-chromosome, suggesting their involvement in transposon control on the inactivating X-chromosome.
Project description:Identification of RNAs associated with the epigenetic activator MLL1 in undifferentiated and differentiated CJ7 mouse embryonic stem cells.
Project description:There are a total of four samples each for this analysis. Each sample consists of the cells grown on three 10 cm culture plates. Each plate should have 2x106 cells for a total of 6x106 cells per sample when all three plates are combined. The first sample is undifferentiated human embryonic stem cells, the second sample is human glutamatergic neurons derived from those human embryonic stem cells, the third sample is undifferentiated human induced pluripotent stem cells and the fourth sample is human glutamatergic neurons derived from those human induced pluripotent stem cells.
