Project description:Interventions: Group 1: Quantitative Expression Analysis of the proteom and gene Expression of Primary Tumor, normal tissue, and metastases Primary outcome(s): Disease associated Proteins and Genes Study Design: Allocation: ; Masking: ; Control: ; Assignment: ; Study design purpose: basic science

| 2431957 | ecrin-mdr-crc

Caenorhabditis elegans

Project description:Global analysis of dauer gene expression in Caenorhabditis elegans

| PRJNA92707 | ENA

Arabidopsis thaliana

Project description:Global gene expression analysis of Arabidopsis ppi2-1 mutant

| PRJNA113557 | ENA

TR accompanied with the study titled A multicenter phase II study of Nivolumab monotherapy in recurrent and/or metastatic gastrointestinal cancer patients with high Tumor Mutation Burden (TMB-H).

Project description:Primary outcome(s): 1. Evaluation of genome abnormality and gene expression by omics analysis of tumor etc. 2. TCR repertoire analysis and RNA expression analysis etc. of T cells in tumor tissue and peripheral blood. 3. Prediction and identification of tumor neo-antigen and evaluation of immunogenicity etc. 4. Analyze ctDNA(16S rRNA PCR) and feces of patients with advanced solid malignancies over time to profile and monitor cancer-related genomic alterations 5. Assessment of the relationship between the analysis above and clinical pathological features or therapeutic efficacy etc.

| 2648665 | ecrin-mdr-crc

Targeted proteome analysis of the central carbon metabolism related enzymes in S. cerevisiae mutant strains

Project description:Expression profiles of 110 the central metabolism-related enzymes were obtained by the selected reaction monitoring (SRM) assay methods using LC-MS/MS from the wild type (BY4742), a GCR2 gene deletion strain, and 29 single-gene deletion strains lacking enzyme genes responsible for central carbon metabolism (including CIT1, ENO1, FBP1, GCR2, GND1, GPD1, GPM2, HOR2, HXK1, HXK2, IDH1, IDH2, IDP1, LPD1, MAE1, MDH1, MDH2, PDA1, PDC1, PFK1, PYC2, RPE1, TAL1, TDH1, TDH2, TDH3, TKL1, TPS1, TPS2, and ZWF1 genes). The central carbon metabolism is strictly controlled by modulation of enzyme expressions to maintain an essential system of living organisms. In this study, metabolic safety mechanisms in the model organism, Saccharomyces cerevisiae, were investigated by direct determination of enzyme expression levels. Targeted proteome analysis of 31 S. cerevisiae wild type and mutant strains revealed that at least 30% of the observed variations in enzyme expression levels could be explained by global regulatory mechanisms. Co-expression analysis revealed that expression levels of enzymes involved in trehalose metabolism and glycolysis changed in a coordinated manner under the control of the transcription factors for global regulation.

2017-03-22 | PXD004714 | JPOST Repository

Canis lupus familiaris

Project description:Global gene expression analysis of canine cutaneous mast cell tumor

| PRJNA217482 | ENA