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Homo sapiens

ABSTRACT: Data expression in alveolar macrophages induced by lipopolysaccharide in humans

OTHER RELATED OMICS DATASETS IN: E-GEOD-40885

PROVIDER: PRJNA175261 | ENA |

REPOSITORIES: ENA

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E-GEOD-40885

Project description:Data expression in alveolar macrophages induced by lipopolysaccharide in humans

2015-09-30 | E-GEOD-40885 | ExpressionAtlas

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Project description:isolated alveolar macrophages

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Data expression in alveolar macrophages induced by lipopolysaccharide in humans

Project description:Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo. In seven healthy humans sterile saline was instilled into a lung segment by bronchoscope, followed by instillation of LPS into the contralateral lung. Six hours later a bilateral bronchoalveolar lavage was performed and whole-genome transcriptional profiling was done (Affymetrix HG-U133 Plus 2.0) on purified alveolar macrophages, comparing cells exposed to saline or LPS from the same individuals.

2012-09-15 | E-GEOD-40885 | biostudies-arrayexpress

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Project description:RNA extracted at 240min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.

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