Genomics

Dataset Information

120

Human betaherpesvirus 5


ABSTRACT: Decoding human cytomegalovirus using ribosome profiling

ORGANISM(S): Human betaherpesvirus 5  

PROVIDER: PRJNA177721 | ENA |

REPOSITORIES: ENA

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Publications


The human cytomegalovirus (HCMV) genome was sequenced 20 years ago. However, like those of other complex viruses, our understanding of its protein coding potential is far from complete. We used ribosome profiling and transcript analysis to experimentally define the HCMV translation products and follow their temporal expression. We identified hundreds of previously unidentified open reading frames and confirmed a fraction by means of mass spectrometry. We found that regulated use of alternative t  ...[more]

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