Project description:The haustorial transcriptomes of Uromyces appendiculatus and Phakopsora pachyrhizi - identification of families of candidate effectors
Project description:A small number of genes (25) expressed differentially with respect to Phakopsora pachyrhizii at V2 growth stage.. We used Affymetrix soybean microarrays to detail the global gene expression underlying host reaction to Phakopsora pachyrhizii and identified differentially expressed genes during this process. Experiment Overall Design: Soybean cv. 5601T plants were grown in greenhouse in six 4-liter pots. Three weeks old plants at V2 growth stage in three out of six pots were inoculated with Phakopsora pachyrhizi and remaining three pots were used as control. Leaf samples from all pots were collected 72 hours after inoculation and RNA was extracted using Tri-Regent. Microarray analysis was performed using Affymetrix platform.
Project description:Affymetrix soybean genome arrays were used to identify genes differentially expressed in the immune resistance response at 6, 12, 24, and 48 hours after inoculation with Phakopsora pachyrhizi isolates TW72-1 or HW94-1 soybean + HW94-1 = resistant; soybean + TW72-1 = susceptible Keywords: time course
