Project description:We used flower bud transcriptomes from Collinsia rattanii and its predominantly outcrossing sister species, C. linearis, to explore the genomic basis of mating system and phenotypic evolution in Collinsia, a self-compatible genus. Transcriptional regulation of enzymes involved in pollen formation may influence floral traits that distinguish selfing and outcrossing Collinsia species through pleiotropic functions. These patterns provide clues about parallel evolution in selfing plants.
Project description:Hymenoscyphus linearis isolate chi01 was grown on potato dextrose broth for 3 weeks. RNA was extracted from the mycelial mat and the library was prepared with TruSeq Stranded mRNA LT Sample Prep Kit and sequenced with HiSeq 2500 (2x100 bp).
