Project description:Assessing relevant molecular differences between human-induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) is important, given that such differences may impact their potential therapeutic use. Controversy surrounds recent gene expression studies comparing hiPSCs and hESCs. Here, we present an in-depth quantitative mass spectrometry-based analysis of hESCs, two different hiPSCs and their precursor fibroblast cell lines. Our comparisons confirmed the high similarity of hESCs and hiPSCS at the proteome level as 97.8% of the proteins were found unchanged. Nevertheless, a small group of 58 proteins, mainly related to metabolism, antigen processing and cell adhesion, was found significantly differentially expressed between hiPSCs and hESCs. A comparison of the regulated proteins with previously published transcriptomic studies showed a low overlap, highlighting the emerging notion that differences between both pluripotent cell lines rather reflect experimental conditions than a recurrent molecular signature. See the Data Processing section of the published paper concerning the bioinformatics pipeline used. PMCID: PMC3261715 PMID: 22108792 Mol Syst Biol. 2011 Nov 22;7:550. doi: 10.1038/msb.2011.84. The quantitative proteomes of human-induced pluripotent stem cells and embryonic stem cells. Munoz J, Low TY, Kok YJ, Chin A, Frese CK, Ding V, Choo A, Heck AJ. Biomolecular Mass Spectrometry and Proteomics Group, Bijvoet Center for Biomolecular Research and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands.

2013-02-01 | PXD000134 | Pride

Homo sapiens

Project description:Expression data from hESCs, hiPSCs and human fibroblasts.

| PRJNA127733 | ENA

Homo sapiens

Project description:Comparison of gene expression profilings between SFEBq differentiation-defective clones and good clones

| PRJNA182178 | ENA

Homo sapiens

Project description:Global expression profile difference of hiPSCs between hESCs and human Urine-Derived Cells

| PRJNA339680 | ENA

Expression data of human induced pluripotent stem cells (hiPSCs), human embryonic stem cells (hESCs) and those differentiated cells.

Project description:We examined hiPSCs, hESCs and those differentiated cells to identify pluripotent signature genes, differentiation marker genes and relationship between expression and phenotypes. Additionally, we performed microarray experiments to examine gene expression in human tissues. This data was used for comparison with hESCs, hiPSCs and those differentiated cells. A total of 22 tissues (bone marrow, cerebellum, colon, cortex, fetal brain, heart, kidney, liver, lung, pancreas, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testes, thymus, thyroid, trachea and uterus) were examined.

2023-05-09 | GSE39144 | GEO

Homo sapiens

Project description:Comparison of gene expression profilings between Mock-WS480, NonV50-OKS-SW480 and 2ndV50-OKS-SW480

| PRJNA360906 | ENA

Homo sapiens

Project description:Comparison of gene expression profilings between parental A549, OSK-A549-Colony and OSK-A549-SN

| PRJNA350784 | ENA