Project description:The following strains of Paenibacillus larvae classified as ERIC I-IV genotypes (pl1-pl4) were used in the study: ERIC I - DSM-7030; ERIC II - DSM-25430; ERIC III - LMG-16252; and ERIC IV - DSM-3615.
The 7 biological replicates (a-g) of the exoprotein fractions prepared from each ERIC genotype were used in the nanoLC-MS/MS analysis.
All 28 samples were consecutively analyzed using an OrbitrapTM FusionTM Tribrid q-OT-IT mass spectrometer.
To improve the protein identification and easy exclude contaminants from the medium, we considered the proteins that could originate from the yeast extract and Mueller-Hinton broth (contains beef fusion and casein peptone-acidic hydrolysate) in analyzing the MS data. Thus, we included the sequences related to Saccharomyces cerevisiae and Bos taurus, respectively, to the list of contaminants in MaxQuant. The contaminants.fasta file is provided. Futhermore, we provide the database (uniprot-paenibacillus+larvae.fasta) used for the identification of P. larvae proteins created of different strains of P. larvae.
The sequences were downloaded of UniProt on 12.03.2019.
Finally, the compressed (zipped) folder "combined" is provided.
The data were analyzed in MaxQuant v1.6.3.4.
