Project description:We treated a homeless man in Iran with a history of squamous cell carcinoma who had ophthalmomyiasis caused by Chrysomya bezziana parasites. This case highlights a much-neglected condition and describes measures to prevent it.
Project description:<i>Chrysomya nigripes</i> (Diptera: Calliphoridae) is a blow fly species of forensic importance. Here we demonstrated the complete mitochondrial genome of this species for the first time. Phylogenetic analyses indicated that entire mitochondrial genome sequences can provide more useful information for distinguishing <i>C. nigripes</i> from the other species.
Project description:Chrysomya pinguis (Walker) (Diptera: Calliphoridae) is an endemic Asiatic blow fly species of forensic importance. Chrysomya pinguis is one of the first species to colonize a corpse, especially in high altitude areas during spring and autumn when the ambient temperature is lower. Despite its potential for forensic investigations to estimate the minimum postmortem interval (PMImin), little is known about the development of C. pinguis. In this study, C. pinguis was collected from the Yangtze River Delta region of China and reared at seven constant temperatures between 16°C and 34°C to investigate the effect of temperature on development duration, accumulated degree hours and larval body length of C. pinguis. Isomorphen and isomegalen diagrams for C. pinguis were generated using the results, and equations describing the variation in larval body length during development and the temperature-induced variation in development time were also obtained. Chrysomya pinguis can complete its life cycle at 16-34°C. The mean (±s.d.) developmental durations of C. pinguis from egg to adult at 16°C, 19°C, 22°C, 25°C, 28°C, 31°C and 34°C were 811.0 ± 3.8, 544.8 ± 2.0, 379.8 ± 1.8, 306.7 ± 2.4, 250.0 ± 2.8, 203.2 ± 2.1 and 185.3 ± 1.6 h, respectively. The mean (±s.e.) developmental threshold temperature D0 and the thermal summation constant K of the whole developmental process of C. pinguis were estimated as 10.88 ± 0.21°C and 4256.50 ± 104.50 degree hours, respectively. This study provides fundamental development data for the use of C. pinguis to estimate PMImin.
Project description:Blow flies are worldwide the most important insects from a forensic point of view. In Thailand, aside from the two most common species, Chrysomya megacephala (F.) and Chrysomya rufifacies (Macquart), Chrysomya chani Kurahashi was also found to be of forensic importance. We present a case of a human female cadaver in its bloated stage of decomposition, discovered at Pachangnoi Subdistrict, northern Thailand. Entomological sampling during the autopsy displayed an assemblage of numerous dipteran larvae. Macroscopic observations showed the coexistence of third instar larvae of the three blow flies C. megacephala, Chrysomya villeneuvi Patton, an unknown blow fly species and one muscid, Hydrotaea sp. The minimum post-mortem interval was estimated to be six days, based on the developmental rate of C. megacephala. The ID of the unknown larva, which is the focus of this report, was revealed later as C. chani by DNA sequencing, using a 1205 bp of cytochrome c oxidase subunit I (COI). The occurrence of C. chani on a human body revealed the need to analyse and describe the morphology of its immature stage, to enable forensic entomologists to identify this fly species in future cases. The morphological examination of the third instar was performed, revealing peculiar characteristics: protuberant tubercles encircling abdominal segments; 9-11 lobes on the anterior spiracle; six prominent pairs of tubercles along the peripheral rim of the eighth abdominal segment; a heavily sclerotized complete peritreme of the posterior spiracles. A key to differentiate the third instar of blow flies of forensic importance in Thailand is provided.