Clostridioides difficile
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ABSTRACT:
PROVIDER: PRJNA185378 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Comparison of the expression profiles of the 630E strain after 8h, 14h and 38h of infection in mouse
| PRJNA185377 | ENA
Project description:Comparison of the expression profiles of the 630E strain and a sigE mutant after 14h of growth
| PRJNA184892 | ENA
Project description:Comparison of the expression profiles of the 630E strain and a sigF mutant after 14h of growth
| PRJNA184893 | ENA
Project description:Transcriptionnal profiling of C. difficile R20291 strain vs a R20291 strain after4h 6h 8h 14h 38h of growth in Vivo
2016-01-31 | GSE35726 | GEO
Project description:The virulence factors of Clostridium difficile have been studied for many years, but the main in vivo pathogenesis processes of this bacterium has to be investigated. Especially, data on the early mechanisms of C. difficile adaptation to the host is not yet available. The objective of our study was to improve the understanding of the pathogenesis of C. difficile by the analysis of the genome-wide temporal expression of C. difficile genes during the first hours of infection. Three groups of 4 axenic mice each were challenged by vegetative cells of the 630 C. difficile strain, and sacrified at 8, 14, and 38 hours post-infection. Pure prokaryotic RNA was obtained from caecal bacteria. Comparative hybridizations on strain 630 microarrays were done using cDNA issued from an 8-hours in vitro culture as control, with a dye-swap protocol for each sample. Normalisation and statistical analysis of all data were done with different functions of the limma package. The pathogenesis of C. difficile could be seen as a result of the successful metabolic adaptation of the bacteria to its host, contributing to its persistence and multiplication, and the coordinate expression of virulence factors. Analysis of our data enlightened some of these aspects. The results support strongly a two-step infection model, since there is, during the course of infection, a significant increase in the toxins expression contrasting with a decreased expression of most of the putative colonization factors. Several paralogs of the HMW S-layer protein are also down-regulated, some of them could be strong candidates as colonisation factors. Bacterial adaptation to the microenvironment of the host is assessed by the regulation of numerous metabolic pathways, i.e., the upregulation of the ethanolamine catabolic operon or the modulation of several PTS systems. Inactivation of some putative virulence factors identified by this methodology will complete this analysis. Transcriptional profiling of the C. difficile 630E strain after 8h, 14h or 38h in TY. Two-condition experiments: 630E strain 14h or 38h in TY vs. 630E strain 8h in TY. 2 biological replicates for each condition in dye swap.
2013-11-04 | E-GEOD-43304 | biostudies-arrayexpress
Project description:Comparison of the expression profiles of 630E JIR8094 strain and a ccpA mutant after 10h of growth in TY.
| PRJNA156171 | ENA
Project description:Comparison of the expression profiles of 630E JIR8094 strain and a ccpA mutant after 10h of growth in TY with 0.5% glucose.
| PRJNA156169 | ENA
Project description:Comparison of the expression profiles of 630E strain after 4h and 10h of growth
| PRJNA142595 | ENA
Project description:Comparison of the expression profiles of the 630E (JIR8094) strain and a prdR mutant (LB-CD8) after 4h growth in TY supplemented with L-proline
| PRJNA183178 | ENA