Project description:Transcriptional profiling of Candida albicans comparing pterostilbene treated cells with untreated cells Wild type Candida albicans strain SC5314 was selected to carry out the expression profile microarray. Two-condition experiment, pterostilbene treated vs. untreated cells. Biological replicates: 3 control, 3 pterostilbene treated, independently grown and harvested. One replicate per array.
Project description:Transcriptional profiling of Candida albicans SC5314 comparing C. albicans grown in RPMI1640 or in RPMI1640 with 100ug/ml AAT. Goal was to determine the effects of AAT on global C. albicans gene expression.
Project description:Purpose: Cellular response of Candida albicans to Aureobasidin A at transcriptomic level. Method: The transcriptome of C. albicans SC5314 upon exposure to 1 µg/ml AbA (0.5X Minimum Inhibitory Concentration) for three hours was analyzed by RNA sequencing (RNA-Seq) on Illumina HiSeq 4000 instrument using the 2 X 150 bp chemistry. Result: Following STAR-alignment of the quality-checked sequence reads against SC5314 reference genome and DESeq2 analysis of the read counts data, we identified 85 differentially-expressed genes between the AbA-treated cells and DMSO-treated controls, with false discovery rate <0.001 and a log2 fold change ≥ 1. Among them, 42 were significantly up-regulated and 43 were significantly down-regulated in AbA-treated cells as compared to the controls. The analysis of biological processes and molecular functions using Gene Ontology (GO) Term Finder of the Candida Genome Database (CGD) showed down-regulation of genes associated with cellular lipid biosynthetic process, and up-regulation of genes associated with regulation of membrane lipid distribution and floppase activity. The genes associated with cell aggregation, biofilm formation and DNA packaging were also affected. Conclusion: In summary, the analysis of transcriptomic data in this study provides beneficial and critical information contributing to our understanding of the cellular response of C. albicans to AbA and its antifungal activity.
