ABSTRACT: The developmental life cycle of the enteric parasite Entamoeba invadens: transcriptome analysis reveals a crucial role for phospholipase D in stage conversion
Project description:RNA-seq was performed on encysting and excysting E. invadens, a parasite of reptiles that is used as a model system to study stage conversion in Entamoeba species, including the human pathogen E. histolytica. The goal of the project was to identify changes to the transcriptome during development, in order to better understand the mechanisms of development.
Project description:mRNA profiles of cysts from Entamoeba invadens treatment with ethanol and treament with 200 nM of Trichostatin A during 24h, using Illumina NextSeq 500
Project description:RNA-seq was performed on encysting and excysting E. invadens, a parasite of reptiles that is used as a model system to study stage conversion in Entamoeba species, including the human pathogen E. histolytica. The goal of the project was to identify changes to the transcriptome during development, in order to better understand the mechanisms of development. RNA was isolated from trophozoites, from encysting parasites (at 8, 24, 48 and 72 hours after transfer to encystation media) and from excysting parasites (2 and 8 hours after induction of excystation), converted to cDNA, and sequenced on a SOLiD platform. RNA-seq data was used to refine gene models, find potential unannotated genes, and identify genes that are developmentally regulated.
Project description:We analyzed ~27nt small RNAs from Entamoeba invadens trophozoites, 24h cysts, 72h cysts, and excysting cells (8h) E. invadens trophozoites were induced to encyst by incubation in low glucose media, and parasites harvested at 0, 24 and 72h. A subset of the 72h cysts were induced to excyst, and parasites harvested at 8h. Total RNA was extracted for each sampleand small RNA libraries constructed and sequenced as below
Project description:To know the role of Rab7 isotypes in enteric protozoan parasite Entamoeba histolytica, one out of nine Rab7 isotypes, Rab7D, was specifically silenced and examined the effect on virulence-related phenotypes. To clarify transcriptomic difference caused by rab7d gene silencing, RNA-seq analysis was conducted.
Project description:To know the role of lysosomal hydrolase receptors in virulecne of enteric protozoan parasite Entamoeba histolytica, eleven cysteine protease binding protein family (CPBF) proteins were specifically silenced and examined the effect on virulence-related phenotypes. Among them, CPBF2 gene silence caused defect in Matrigel invasion. To clarify transcriptomic difference in CPBF2 gene silenceing strain, RNA-seq analysis was conducted.
