Project description:The current study aims to study change in gene expression profile of miltefosine resistance L. donovani with respect to miltefosine responsive L. donovani. Agilent one-color experiment,Organism: Leishmania donovani, Microarray GeneExpression 8x15k designed by Genotypic Technology Private Limited. (AMADID: 035638)
Project description:Differential gene expression analysis was carried out in clinical isolates of Leishmania donovani (L. donovani) parasite derived from VL patient before miltefosine treatment, post treatment and patient that relpased after miltefosine treatment
Project description:The current study aims to study change in gene expression profile of miltefosine resistance L. donovani with respect to miltefosine responsive L. donovani.
Project description:Gene expression analysis was carried out between experimental miltefosine resistant L.donovani (adapted to 30ug/ml miltefosine drug pressure) and its corresponding wild type miltefosine senstive parasite 3 biological replicates of lab generated miltefosine resistant Leishmania donovani (K417 M30) were compared with corresponding wild type sensitive parasite (K417WT)
Project description:Murine bone marrow derived macrophages were infected with Leishmania major or Leishmania donovania promastigotes, allowed to phagocytose latex beads or not treated. Gene expression profiles were compared to identify i) the effect of Leishmania infection; ii) the differences in effects between L. major and L. donovani; and iii) the effect of pahgocytosis of latex beads.
Project description:In this study, we examined the transcriptome of Leishmania donovani promastigotes and axenic amastigotes to identify differentially regulated mRNAs utilizing the serial analysis of gene expression Keywords: stage differentiation; axenic amastigotes
Project description:Histone acetylations are known to impact gene transcription. Here, we have attempted to examine the role of Leishmania donovani histone acetyltransferase HAT4 in regulating global gene expression. The transcriptome of HAT4-null promastigotes (Samples 2A, 2B) have been compared with the transcriptome of wild-type Leishmania promastigotes (Samples 1A, 1B) and fold change in gene expression with respect to the control wild-type has been determined. DNA microarray analysis was carried out with biological replicates, using RNA isolated from logarithmically growing promastigotes in two separate experiments. Bioanalyzer profiles of the isolated RNA samples were used to assess purity and integrity of the isolated RNA. Sample 1A and sample 1B are analyses carried out using RNA isolated from wild-type Leishmania donovani promastigotes. Sample 2A and sample 2B are analyses carried out using RNA isolated from HAT4-null promastigotes.
Project description:Histone acetylations are known to impact gene transcription. Here, we have attempted to examine the role of Leishmania donovani histone acetyltransferases HAT4 and HAT2 in regulating global gene expression. The transcriptome of HAT4-null promastigotes (Samples 2A, 2B) and HAT2-heterozygous knockout promastigotes (Samples 3A, 3B) have been compared with the transcriptome of wild-type Leishmania promastigotes (Samples 1A, 1B) and fold change in gene expression with respect to the control wild-type has been determined.
