Project description:The Clostridium beijerinckii NCIMB 8052 wild-type culture was monitored from exponential growth to stationary phase. During this period the culture underwent a shift from acidogenesis to solventogenesis. Acetone and butanol production was initiated with the onset of the solventogenic phase. Using DNA microarray changes in gene expression were examined during the transitional period. RNA samples were taken from Clostridium beijerinckii NCIMB 8052 wild-type fermentation culture at individual time points during the acidogenic phase and the solventogenic phase. The samples were used for microarray hybridization.
Project description:The Clostridium beijerinckii NCIMB 8052 wild-type culture was monitored from exponential growth to stationary phase. During this period the culture underwent a shift from acidogenesis to solventogenesis. Acetone and butanol production was initiated with the onset of the solventogenic phase. Using DNA microarray changes in gene expression were examined during the transitional period.
Project description:Crystalline chitin is a kind of high molecular-weight polymers which is difficult to degrade. N-acetylglucosamine and chitooligosaccharides (COSs) are important agriculture, medicine, cosmetics and food resources. Conversion of crystalline chitin to GlcNAc and COSs using recombinant chitinases is an environmentally compatible, reproducible and products controllable method. Here, we report the ability of Pseudoalteromonas flavipulchra DSM 14401 to degrade crystalline.