Project description:Campylobacter jejuni is the most prevalent cause of foodborne bacterial enteritis worldwide. This study aims at the characterisation of pathomechanisms and signalling in Campylobacter-induced diarrhoea in the human mucosa. During routine colonoscopy, biopsies were taken from patients suffering from campylobacteriosis. RNA-seq of colon biopsies was performed to describe Campylobacter jejuni-mediated effects. Mucosal mRNA profiles of acutely infected patients and healthy controls were generated by deep sequencing using Illumina HiSeq 2500. This data provide the basis for subsequent upstream regulator analysis. Overall design: Colon mucosa mRNA profiles from 4 acutely infected patients and 6 healthy controls were generated by paired end sequencing using Illumina HiSeq 2500
Project description:Expression arrays comparing Campylobacter jejuni NCTC11168 during growth in the cecum of germ-free C57 BL/6 IL-10 knockout mice to C. jejuni NCTC11168 during growth in Bolton broth. Overall design: Four biological replicates comparing C. jejuni NCTC11168 growth in vivo to in vitro. Two biological replicates were dye swaps.
Project description:Campylobacter jejuni is the leading cause of campylobacteriosis in the developed world. Although most cases are caused by consumption of contaminated meat, a significant proportion is caused by consumption of contaminated water. Some C. jejuni isolates are better than others at surviving in water, which suggests that these strains are better adapted to transmission by water than others. The aim of this study is to investigate this phenomenon further. CFU counts and viability assays showed that strain 81116 survives better than strain 81-176 in a defined freshwater medium at 4°C. Comparative transcriptomic profiling using microarray revealed that these strains respond differently to water. This series presents the transcriptome of strain 81116 in water. Overall design: Campylobacter jejuni was first cultured on TSA-Blood plate at 42 °C for 2 days. C. jejuni was suspended in 100 ml of Fraquil or Brucella broth at an OD600 of 1 in triplicates and washed three times with either Fraquil or Brucella broth. The suspensions were then incubated at 4°C for 4h. RNA was extractedl, labelled, and hybridized on microarrays, with labeled gDNA as reference channel.