Project description:Gene expression profiles of Homarus americanus larvae exposed to five endosulfan concentrations were compared to the control to identify genes that showed significant changes in expression as a result of exposure. There was a significant dose response effect during the endosulfan experiment and changes in gene expression were seen at high endosulfan concentrations that could caused developmental delays and mortality
Project description:Gene expression profiles of Homarus americanus larvae exposed to five endosulfan concentrations were compared to the control to identify genes that showed significant changes in expression as a result of exposure. There was a significant dose response effect during the endosulfan experiment and changes in gene expression were seen at high endosulfan concentrations that could caused developmental delays and mortality A six condition experiment: control vs 5 endosulfan concentrations. Biological replicates: 6. Array replicates: 1
Project description:Transcriptional profiling of larval stage I - IV of Homarus americanus, for assessment of stage specific developmental gene expression. Individuals from each stage were gathered in July of 2009 and 2010. A reference design was used to permit comparison of all stages. Study aims included assessment of global gene expression of larval development of healthy larvae, and identifying novel molecular pathways involved in H. americanus development.
Project description:Transcriptional profiling of larval stage I - IV of Homarus americanus, for assessment of stage specific developmental gene expression. Individuals from each stage were gathered in July of 2009 and 2010. A reference design was used to permit comparison of all stages. Study aims included assessment of global gene expression of larval development of healthy larvae, and identifying novel molecular pathways involved in H. americanus development. Four condition experiment, stage I - IV individuals: 10 biological replicates per stage. Stages collected independantly from each other; sampling conducted over 2 seasons. Reference sample prepared from pooled RNA of 4 individuals per stage.
Project description:The American lobster, Homarus americanus, is not only of considerable economic importance but has also emerged as a pivotal model in neuroscience research. Neuropeptides, an important class of cell-to-cell signaling molecules, play crucial roles in a wide array of physiological and psychological processes. In light of the recently sequenced high-quality draft genome of the American lobster, our study sought to profile the neuropeptidome in this model organism. Employing advanced mass spectrometry techniques alongside functional genomic analysis, we identified 24 neuropeptide precursors and 101 unique mature neuropeptides in Homarus americanus. Intriguingly, 67 of these neuropeptides were discovered for the first time. Our findings offer a comprehensive overview of the peptidomic attributes of the lobster's nervous system and highlight the tissue-specific distribution of these neuropeptides. Collectively, this research not only enriches our understanding of the neural complexities of the American lobster but also sets a foundational basis for future investigations into the functional roles that these peptides play in crustacean species.
