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Aggregatibacter actinomycetemcomitans strain:624

ABSTRACT: Aggregatibacter actinomycetemcomitans strain:624 Transcriptome or Gene expression

PROVIDER: PRJNA203447 | ENA |

REPOSITORIES: ENA

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Aggregatibacter actinomycetemcomitans strain:624

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Project description:The two-component system qseBC is a putative prokaryotic adrenergic receptor. In Aggregatibacter actinomycetemcomitans (Aa), it was shown that the qseBC responds significantly to the presence of both catecholamines and ferrous iron. In addition, growth is significantly increased in the presence of both. Therefore, we performed a custom Aa microarray in order to determine the effects of catecholamines and catecholamines and iron (CAT-Fe) on Aa. Aggregatibacter actinomycetemcomitans was grown in triplicate in a chemically defined media (CDM) supplemented with either 100uM of ferrous chloride or 50uM of norepinephrine or both. At mid-log, RNA was harvested using the Qiagen Lipid Tissue Minikit.

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Transcriptome analysis of Aggregatibacter actinomycetemcomitans strains isolated from the same individual during short term persistent infections using a pan-genome microarray

Project description:To characterize the differences in pattern of gene expression between different pairs of Aggregatibacter actinomycetemcomitans strains (SCC393 vs A160, SCC1398 vs SCC4092, and S23A vs I23C), where each pair was isolated from an individual over time. The microarray was used to examine the transcriptome of Aggregatibacter actinomycetemcomitans strains SCC393, A160, SCC1398, SCC4092, S23A, and I23C, where biological triplicates were produced for strains SCC1398, SCC4092, S23A, and I23C and biological duplicates were produced for strains SCC393, and A160

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