Project description:The transformation of endosymbiotic bacteria into genetically integrated organelles was central to eukaryote evolution. During organellogenesis, control over endosymbiont division, proteome composition, and physiology largely shifted from endosymbiont to nucleus. However, the order and timing of events underpinning this major evolutionary transition are poorly understood. Here, we identified by protein mass spectrometry seven nucleus-encoded proteins that are targeted to the endosymbiont.
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from uninfected and infected samples at 3 time points (3, 10 and 20 days). Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans
Project description:Transcriptome analysis of Wigglesworthia glossinidia endosymbiont derived from control samples with or without parasite contact at 10 days. Expression profiling by array - Wigglesworthia glossinidia endosymbiont of Glossina morsitans morsitans RNAs are a mix of Wigglesworthia, Sodalis and glossina. RNAs were extracted from 8 samples including 2 conditions (with 4 replicates per condition).
Project description:We report the application of Cappable-seq to selectively enrich prokaryotic endosymbiont transcripts from mixed host-symbiont total RNA.
Project description:Hfq-dependent transcriptional alterations in the nitrogen-fixing endosymbiont S. meliloti. Comparison: S. meliloti 1021 strain Vs S. meliloti 1021Dhfq (containing a deletion of the hfq ORF).