Project description:mRNA profiles of control Human Trophoblast Stem cell (HTS) and TEAD4 knock down HTS cells were generated by deep sequencing, in triplicate, using Illumina NovaSeq 6000 platform. TEAD4 Knock down in HTS cells were confirmed by RT-PCR analysis and immuno staining.
Project description:Differential mRNA expression patterns were seen in GSC272-vector compared to GSC272-POSTN shRNA tumors. We used microarrays to POSTN regulated gene expression in glioma stem cells. Gene expression comparisons were shown between: 1. mRNA control from GSC272 glioma stem cells implanted mouse brain tumor at 7 weeks after implantation, and 2. mRNA deplection of POSTN from knock down of POSTN of GSC272 glioma stem cells implanted mouse brain tumor at 7 weeks after implantation.
Project description:To better understand the role of MbovGdpP in cellular processes, the expression profiles of differentially expressed genes between M. bovis wild type strain HB0801 and MbovGdpP knock-out mutant were determined by RNA-sequencing (RNA-seq). Differential gene expression analysis identified up to 161 genes with significant changes in T6.290 (|log2 fold change| > 1.5 and p < 0.05), with 74 mRNA up-regulated and 87 down-regulated. Transcriptomic analyses revealed the biological importance of MbovGdpP.
