Project description:Hyphopichia burtonii has much stronger osmotolerance than model Saccharomyces cerevisiae and other yeast. The transcriptomic changes by RNA-seq analysis in several hyperosmotic stresses and YPD condition as control were investigated, expecting changes in the expression patterns of genes that do not exist or change in other yeast.
Project description:The goal and objectives of this experiment are to find out the transcriptomic changes and differences when the two halotolerant yeast H. burtonii and H. pseudoburtonii isolated from Nuruk were exposed to high salt conditions over time. So the RNA-seq anaylsis was perfomred by setting up each time points (0, 5, 15, 30 min) The sequencing data of RNAs obtained from yeast cells cultured in YPD + 1M NaCl at each time were analyzed using YPD condtions as acontrol
Project description:Transcriptome data of Hyphopichia burtonii and Hyphopichia pseudoburtonii when exposed to high osmosterss (1M NaCl) condtion at several time points.
Project description:Investigation of whole genome gene expression level changes in Spathaspora passalidarum NRRL Y-27907 grown aerobically in xylose, compared to the same strain grown aerobically in glucose. A six array study using total RNA recovered from three separate cultures of Spathaspora passalidarum NRRL Y-27907 grown in glucose and three separate cultures of Spathaspora passalidarum NRRL Y-27907 grown in xylose. Each array measures the expression level of 362,487 probes (average probe length 54.5 +/- 4.0 nt) tiled across the Spathaspora passalidarum NRRL Y-27907 genome with a median spacing distance of 29 nt. During data processing, probes are filtered to include only those probes corresponding to annotated protein-coding genes.