Project description:Because pink1-mutant flies exhibit a global shutdown of protein synthesis, we decided to measure the levels of individual proteins in adult flies through quantitative proteomics.
Project description:Hemolymph samples were collected from 80-100 control or apoptosis-deficient male flies in three biological replicates. Hemolymph samples were subjected to trypsin-digestion. Digested-peptides were desalinated and purified using GL-tip SDB. Purified peptides were subjected to LC-MS/MS analysis.
