Project description:Hyphopichia burtonii has much stronger osmotolerance than model Saccharomyces cerevisiae and other yeast. The transcriptomic changes by RNA-seq analysis in several hyperosmotic stresses and YPD condition as control were investigated, expecting changes in the expression patterns of genes that do not exist or change in other yeast.
Project description:The goal and objectives of this experiment are to find out the transcriptomic changes and differences when the two halotolerant yeast H. burtonii and H. pseudoburtonii isolated from Nuruk were exposed to high salt conditions over time. So the RNA-seq anaylsis was perfomred by setting up each time points (0, 5, 15, 30 min) The sequencing data of RNAs obtained from yeast cells cultured in YPD + 1M NaCl at each time were analyzed using YPD condtions as acontrol
